Recombinant SUMO4 Antibody (Ab191339) - Western Blot All lanes: Recombinant SUMO4 Antibody (Ab191339) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 16 kDa Exposure time: 3.4 s
Recombinant SUMO4 Antibody (Ab191339) - IF/ICC Immunofluorescent analysis of SUMO4 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant SUMO4 Antibody (Ab191339) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
ZJ24F1215685
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