Flow Cytometry: Human peripheral blood lymphocytes
偶联
FITC
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
克隆类型
单克隆抗体
Format
Whole IgG
纯化方法
Protein G purified
浓度
5 μL/test
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5% triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Unstimulated Human PBMC were used as control (Left panel).
IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5% triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Left panel - Mouse IgG (FITC) (Ab180307).
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
ZJ25F0825217
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