可作为肠上皮细胞(IECs)和粘膜上皮细胞内淋巴细胞(IELs)之间的物理亲和相互作用分子,提供免疫屏障,作为抵御粘膜感染的第一道防线。在胚胎干细胞增殖和分化中发挥作用。上调 FABP5、MYC 以及细胞周期蛋白 A 和 E 的表达。
宿主种属
小鼠(Mouse)
特异性
EpCAM
种属反应性
人(Human)
免疫原
Recombinant protein corresponding to Human EpCAM
阳性对照
WB: HT-29 whole cell lysate IF/ICC: HT-29 cells
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
CD04/3H2
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
图片
EpCAM Mouse mAb (Ab191888) - Western Blot All lanes: EpCAM Mouse Antibody (Ab191888) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 32, 39 kDa Exposure time: 60.0 s
EpCAM Mouse mAb (Ab191888) - ICC/IF IF analysis of EpCAM (Green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with EpCAM Mouse mAb (Ab191888) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.