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EpCAM Mouse mAb
货号:
Ab191888
级别和纯度:
无载体 ?
无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。
ExactAb™ ?
ExactAb™ —— 阿拉丁强调精准和验证质量的抗体系列。适用于可靠、特异性经验证的抗体应用。
无叠氮钠 ?
无叠氮(Azide Free)—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。
已验证 ?
已验证级 —— 发布前在特定应用中确认性能。当需要试剂按声称工作的书面保证时使用。
高性能 ?
高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。
PBS Only ?
仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。
≥95%(SDS-PAGE)
1.0 mg/mL
概述
Mouse anti Human EpCAM Antibody, clone CD04/3H2, could be used for WB, IF/ICC, ELISA and so on.
规格
别名
上皮细胞粘附分子|Anti-EpCAM抗体|EpCAM小鼠单克隆抗体|CD326小鼠单克隆抗体|EpCAM抗体|EPCAM/TROP1抗体|EpCAM 小鼠单克隆抗体|EpCAM 小鼠单抗
英文别名
17 1A antibody|323/A3 antibody|Adenocarcinoma associated antigen antibody|Adenocarcinoma-associated antigen antibody|Antigen identified by monoclonal antibody AUA1 antibody|AUA1 antibody|CD326 antibody|CD326 antigen antibody|Cell surface glycoprotein Trop 1 antibody|Cell surface glycoprotein Trop 2 antibody|Cell surface glycoprotein Trop-1 antibody|CO17 1A antibody|CO17A antibody|DIAR5 antibody|EGP 2 antibody|EGP antibody|EGP2 antibody|EGP314 antibody|EGP40 antibody|Ep CAM antibody|Ep-CAM antibody|EPCAM antibody|EpCAM1 antibody|Epithelial cell adhesion molecule antibody|Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody|Epithelial cell surface antigen antibody|Epithelial cellular adhesion molecule antibody|Epithelial glycoprotein 1 antibody|Epithelial glycoprotein 314 antibody|Epithelial glycoprotein antibody|ESA antibody|GA733 1 antibody|GA733 2 antibody|GA733-2 antibody|gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody|gp4 antibody|hEGP 2 antibody|hEGP314 antibody|HNPCC8 antibody|Human epithelial glycoprotein 2 antibody|KS 1/4 antigen antibody|KS1/4 antibody|KSA antibody|Ly74 antibody|Lymphocyte antigen 74 antibody|M1S 1 antibody|M1S2 antibody|M4S1 antibody|Major gastrointestinal tumor associated protein GA733 2 antibody|Major gastrointestinal tumor-associated protein GA733-2 antibody|mEGP314 antibody|Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody|Membrane component, chromosome 4, surface marker 1 antibody|Membrane component, chromosome 4, surface marker antibody|MIC18 antibody|MK 1 antibody|Protein 289A antibody|TACD1 antibody|TACSTD1 antibody|TROP1 antibody|Tumor associated calcium signal transducer 1 antibody|Tumor associated calcium signal transducer 2 precursor antibody|Tumor-associated calcium signal transducer 1 antibody|EpCAM Monoclonal antibody|Anti-EpCAM antibody|EpCAM/TROP1 Antibody|Mouse anti-EpCAM antibody|Human EpCAM/TROP-1 Antibody|Mouse anti-human EPCAM|Mouse Anti CD326 mAb
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理
可作为肠上皮细胞(IECs)和粘膜上皮细胞内淋巴细胞(IELs)之间的物理亲和相互作用分子,提供免疫屏障,作为抵御粘膜感染的第一道防线。在胚胎干细胞增殖和分化中发挥作用。上调 FABP5、MYC 以及细胞周期蛋白 A 和 E 的表达。
免疫原
Recombinant protein corresponding to Human EpCAM
阳性对照
WB: HT-29 whole cell lysate IF/ICC: HT-29 cells
储存与运输
稳定性与储存
4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
图片
Western Blot: EpCAM Mouse mAb (Ab191888)
EpCAM Mouse mAb (Ab191888) - Western Blot All lanes : EpCAM Mouse Antibody (Ab191888 ) at 1/1000 dilutionSamples : Lysates at 20 µg per laneSecondary : Goat Anti-Mouse IgG H&L (HRP) (Ab179001 ) at 1/20000 dilutionPredicted band size: 20 kDa Observed band size: 32, 39 kDa Exposure time: 60.0 s
ICC/IF: EpCAM Mouse mAb (Ab191888)
EpCAM Mouse mAb (Ab191888) - ICC/IF IF analysis of EpCAM (Green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with EpCAM Mouse mAb (Ab191888 ) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
应用
WB
1/1,000 – 1/10,000; Predicted molecular weight: 34.9 KDa
高级数据
关联靶点(人)
作用机制
作用机制
Action Type
target ID
Target Name
Target Type
Target Organism
Binding Site Name
参考文献
质检证书(CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
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