Diubiquitin antibody | FAT10 antibody | GABBR1 antibody | UBD 3 antibody | Ubd antibody | UBD_HUMAN antibody | Ubiquitin D antibody | Ubiquitin like protein FAT10 antibody | Ubiquitin-like protein FAT10 antibody | Diubiquitin | FAT10 | FAT10diubiquitin |
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN).
Post-translational: Can be acetylated.
宿主种属
兔(Rabbit)
特异性
UBD
种属反应性
人(Human),小鼠(Mouse)
免疫原
A synthetic peptide derived from human FAT10 (AA 100-165).
阳性对照
WB: Hep G2, MCF7, SH-SY5Y and NIH/3T3 cell lysates. IF/ICC: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant FAT10 Antibody (Ab215127) - Western Blot All lanes: Recombinant FAT10 Antibody (Ab215127) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 18 kDa Observed band size: 24 kDa Exposure time: 120.0 s
Recombinant FAT10 Antibody (Ab215127) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant FAT10 Antibody (Ab215127) at 1/50 dilution.
ZJ25F0218604
危险品化学品经营许可证（带存储）