Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
宿主种属
兔(Rabbit)
特异性
MLH1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human MLH1 (AA 350-756)
阳性对照
WB: HeLa, A549, Jurkat, A431 and C6 cell lysates. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, pH 7.4
防腐剂
0.01% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(24个月);收货后建议分装,避免反复冻融。
图片
Recombinant MLH1 Antibody (Ab309267) - Western Blot
All lanes: Recombinant MLH1 Antibody (Ab309267) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 42.0 s
Recombinant MLH1 Antibody (Ab309267) - Western Blot
All lanes: Recombinant MLH1 Antibody (Ab309267) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 42.2 s
Recombinant MLH1 Antibody (Ab309267) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant MLH1 Antibody (Ab309267) (red), and DAPI (blue).