C2627056计算溶液所需的质量、体积或浓度。
羧肽酶B
有货
为什么选择此级别
EnzymoPure™,天然 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
细胞定位：Cytoplasmic vesicle. Secreted.
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 7.9, and the pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., 1,10-phenanthroline.
The enzyme is chromatographically purified and supplied as a solution in 100 mM sodium chloride. Chymotrypsin and trypsin 0.02%.
Carboxypeptidase B (CPDB) is a metallocarboxypeptidase that catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides:
Peptidyl-L-lysine(-L-basic amino acid) + H2O --> Peptide + L-lysine(L-basic amino acid)
Carboxypeptidases are secreted as zymogens by the acinar cells of the pancreas. The zymogens are activated by trypsin in the small intestine.
Carboxypeptidase B Assay
Method
Activity is measured by the spectrophotometric method where the reaction velocity is determined by an increase in absorbance at 254 nm resulting from the hydrolysis of hippuryl-L-arginine. One unit causes the hydrolysis of one micromole of hippuryl-L-arginine per minute at 25°C and pH 7.65 under the specified conditions.
Reagents
0.025 M Tris⋅HCl buffer, pH 7.65 containing 0.1 M sodium chloride
0.001 M Hippuryl-L-arginine in 0.025 M Tris⋅HCl pH 7.65 containing 0.1 M sodium chloride
Enzyme
Dilute stock solution with reagent grade water to a concentration of 1-5 units/ml.
Procedure
Set spectrophotometer at 254 nm and 25°C.
Pipette 2.9 ml of substrate into cuvette and incubate in spectrophotometer at 25°C for 3-4 minutes to reach temperature equilibration and establish blank rate, if any. Add 0.1 ml of diluted enzyme and record increase in A254 for 3-4 minutes. Determine ΔA254/minute from the initial linear portion of the curve.
Calculation
where 0.349 is the extinction coefficient of hippuric acid formed during the reaction
规格
产品名称
羧肽酶B
别名
肽基-L-赖氨酸(L-精氨酸)水解酶 | 肽酰 - L - 赖氨酸 (L - 精氨酸) 水解酶 | 精蛋白酶
英文别名
DTXSID801009277 | FT-0623486 | Carboxypeptidase B from human pancreas, 50-55 units/mg protein carboxypeptidase B | 4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobu
规格或纯度
EnzymoPure™,天然,≥170 units/mg protein;来源于猪胰腺
生化机理
羧肽酶B是一种蛋白水解酶，能够快速水解肽键，从而从肽和蛋白质中释放某些羧基末端碱性氨基酸。其分子量为34,300±600 Da。其每摩尔含有一克不可透析的锌原子。金属螯合剂1,10-菲咯啉、8-羟基喹啉-5-磺酸、和2,2′-联吡啶可抑制该酶活性。
生物活性
≥170 U/mg protein
种属
猪(Pig)
序列
Belongs to the peptidase M14 family.
来源
天然
预测分子量
34.7 kDa
CAS编号和信息
9025-24-5
酶学委员会编号
EC 3.4.17.2
分子类型
酶
储存与运输
物理形态
液体
储存缓冲液
100mM sodium chloride.
浓度
≥170 units/mg protein;来源于猪胰腺
储存条件
-20°C储存
运输条件
超低温运输
单位定义
One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65.
技术文档
高级数据
三维结构
交互式化学结构模型
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技术文档和文章
溶液计算器
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