胆固醇氧化酶来源于微生物

CAS: 9028-76-6 货号: C139686 EC号: 232-842-1

20篇引用文献 COA

有货

级别和纯度: EnzymoPure™ ? ＞10U/mg

Accession #

P12676

应用

不同环境中的微生物代谢；类固醇降解

生物活性

≥10 U/mg enzyme powder

规格

库存

价格

数量

1KU

C139686-1KU

现货

¥799.90

5KU

C139686-5KU

现货

¥2,599.90

请输入您要添加的产品数量

大包装 / 定制

♡ 收藏夹 ⇅ 比较 ⎙ 打印页面

Skip to the end of the images gallery

Skip to the beginning of the images gallery

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章引用文献溶液计算器

🧪

为什么选择此级别

EnzymoPure™ 级，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-20°C储存。超低温运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 20 篇同行评审文献引用。

概述

This product is a cholesterol oxidase preparation extracted from bacteria.

Catalysis

Cholesterol + O₂ → Cholest-4-en-3-one + H₂O₂

Specification and Preparation

Activity: ≥10 U/mg enzyme powder

Contaminant: Catalase activity ≤ 0.5%

Appearance: Light yellow to yellowish orange powder, lyophilized

Additives: Saccharose

Characteristics

Molecular weight: 64,000 (SDS-PAGE)

Isoelectric point: 4.7

Km: 3.0×10⁻⁵ M

Optimum pH: 7.0

pH stability: 5.0–9.0 (37°C, 1hr)

Optimum temperature: 45–50°C

Thermal stability: up to 40°C (4hr, dissolved in purified water)

Activator: Sodium cholate

Stabilizer: Sodium cholate

Application: Used for enzymatic determination of cholesterol in serum by coupling with cholesterol esterase in clinical diagnosis.

Safe Handling

1. Do not inhale.

2. In case of direct contact with skin or eyes, immediately wash or rinse with plenty of water.

General properties

The following results demonstrate the activities of enzyme solution prepared in various buffers.

Enzyme activity may vary under different experimental conditions.

Assay method

Principle

The appearance of quinoneimine dye formed by coupling with 4-aminoantipyrine and phenol is measured at 500 nm byspectrophotometry.

Unit Definition

One unit is defined as the enzyme quantity which oxidizes one µ mole of cholesterol per minute under the conditions described below.

Reagents

A. 4-Aminoantipyrine solution (5 mg/ml deionized water)

B. Triton X-100 stock solution (50 mg/ml deionized water)

C. Phenol solution (50 mg/ml deionized water)

D. 0.1M Phosphate buffer (KH₂PO₄-NaOH, pH 7.0)

E. 0.1% Triton-X Solution

Dilute Triton X-100 stock solution (B) to 0.1% concentration with deionized water.

F. Aminoantipyrine-phenol solution

Mix 5.5 ml of 4-Aminoantipyrine solution (A), 5.5 ml of Phenol solution (C) and 29 ml of TritonX-100 stock solution (B), then fill up to 500 ml with 0.1 M Phosphate buffer (D). After preparation, release the lid of the container and allow the solution to stand under refrigeration for 24 hours before use. (Can be used for 3 months in light-shielded and refrigerated storage)

G. Substrate solution

Weigh 23 mg of cholesterol and dissolve in 10 ml of 2-propanol. (Can be used for 5 days at room temperature)

H. Peroxidase solution

Dissolve 330 units of Peroxidase in 2ml of chilled 0.1 M Phosphate buffer (D). (Can be used for 2 weeks at 2-8℃)

I. Working solution

Mix 1ml of Substrate solution (G) and 58 ml of Aminoantipyrine-phenol solution (F), then add 1 ml of Peroxidase solution (H) and mix. (Can be used for 18 days in light-shielded and refrigerated storage)

J. Enzyme solution

Weigh 20 mg of Cholesterol Oxidase and dissolve in 5 ml of deionized water and keep the solution at 37±0.5℃ in a water bath for 4 hours. Then, after cooling down in an ice bath, dilute the solution with chilled 0.1% Triton X-100 solution (E). Enzyme solution should be prepared so that the value of ΔOD/minute becomes in the range of 0.020±0.005.

Procedure

Put 3ml of Working solution (I) into a quartz cell (d = 10 mm) and keep at 37±0.5℃ for 10 minutes. Then, pipette 0.1 ml of Enzyme Solution (J) into the quartz cell and mix well immediately. Keep the reaction mixture at 37±0.5℃. Exactly at 3 minutes and 6 minutes after the addition of Enzyme solution (J), measure the absorbances of the reaction mixture at 500 nm (A3 and A6). As a blank, pipette 0.1% Triton X-100 solution (E) into another quartz cell instead of Enzyme solution (J) and take the same procedure described above (Ab3 and Ab6).

Calculation

3: Reaction time

13.6: Millimolar absorption coefficient of quinoneimine dye

2: Conversion factor (1 mole of quinoneimine dye equivalent to 2 mole of cholesterol)

3.1: Volume of the reaction mixture

0.1: Volume of Enzyme solution

n: Dilution factor of Enzyme solution

规格

产品名称

胆固醇氧化酶来源于微生物

别名

胆固醇：氧氧化还原酶

英文别名

规格或纯度

EnzymoPure™, ＞10U/mg

生化机理

Cholesterol oxidases are secreted bacterial bifunctional enzymes that catalyze the first two steps in the degradation of cholesterol. The enzyme catalyzes the oxidation of the 3beta-hydroxyl group to a keto group, and the isomerization of the double bond in the oxidized steroid ring system from the Delta(5) position to the Delta(6) position (cf. EC 5.3.3.1).

生物活性

≥10 U/mg enzyme powder

Accession #

P12676

作用类型

抑制剂

CAS编号和信息

9028-76-6

酶学委员会编号

1.1.3.6

分子类型

酶

储存与运输

应用

不同环境中的微生物代谢；类固醇降解

物理形态

冻干(Lyophilized)

浓度

＞10U/mg

储存条件

-20°C储存

运输条件

超低温运输

单位定义

在 pH 7.0、37℃条件下，每分钟催化氧化 1 μmol 胆固醇所需的酶量，定义为 1 个胆固醇氧化酶活力单位（U）。

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到15个结果

批号(Lot Number)	证书类型	货号
C2513310	分析证书	C139686
C2513319	分析证书	C139686
G2627196	分析证书	C139686
G2627199	分析证书	C139686
G2408510	分析证书	C139686
J2217288	分析证书	C139686
G2408506	分析证书	C139686
G2408503	分析证书	C139686
C2414048	分析证书	C139686
G2201333	分析证书	C139686
G2201334	分析证书	C139686
J2217298	分析证书	C139686
H2124193	分析证书	C139686
H2124194	分析证书	C139686
K1919044	分析证书	C139686

显示更多⌵

技术文档和文章

生物酶催化技术及应用

目录：技术文章

探索主题:

酶催化剂生物催化技术

Biological enzyme catalysis technology and application

目录：技术文章

探索主题:

Enzyme Catalyzer Biocatalytic technology

石墨烯生物传感器的信号转换机制：从分子识别到电学/光学信号读出与材料选型

目录：技术文章

探索主题:

石墨烯生物传感器

Signal Transduction Mechanism of Graphene Biosensors: From Molecular Recognition to Electrical/Optical Signal Readout and Materials Selection

目录：技术文章

探索主题:

Graphene Biosensor

酶偶联显色反应中的辅助酶分工：变旋酶、氧化酶、过氧化物酶与脱氢酶

目录：技术文章

探索主题:

过氧化物酶氧化酶显色底物结果判读酶偶联显色反应辅助酶变旋酶脱氢酶

Functional Roles of Auxiliary Enzymes in Enzyme-Coupled Colorimetric Reactions: Mutarotase, Oxidases, Peroxidases, and Dehydrogenases

目录：技术文章

探索主题:

Peroxidase Chromogenic Substrate result interpretation Enzyme-Coupled Colorimetric Reaction Auxiliary Enzyme Mutarotase Oxidase Dehydrogenase

此产品的引用文献

如何在您的发表作品中引用阿拉丁的产品和网站? 引用文献检索

引用文献

1. Chu Fenghong, Feng Yuan, Hu Anduo, Dai Caihong, Qi Yue, Bian Zhenglan. (2023) Cholesterol biosensing based on hydrogel optical fiber immobilization with cholesterol oxidase. OPTIK, [10.1016/j.ijleo.2023.170995]
2. Ke Yang, Guoning Chen, Lu Wang, Miao Guo, Jiameng Xu, Yirong Ma, Zhimin Luo, Aiguo Zeng, Qiang Fu. (2023) Zeolitic-imidazolate framework (ZIF-8)-based immobilized multi-enzymes integrated with a colorimetric sensor for cholesterol assay. NEW JOURNAL OF CHEMISTRY, 47 (8): (4103-4112). [10.1039/D2NJ05747F]
3. Ying Li, Shujuan Li, Min Bao, Linqun Zhang, Carlo Carraro, Roya Maboudian, Anran Liu, Wei Wei, Yuanjian Zhang, Songqin Liu. (2021) Pd Nanoclusters Confined in ZIF-8 Matrixes for Fluorescent Detection of Glucose and Cholesterol. ACS Applied Nano Materials, [10.1021/acsanm.1c01712]
4. Yanlei He, Ning Li, Weikang Li, Xianxi Zhang, Xiao Zhang, Zhenxue Liu, Qingyun Liu. (2020) 5,10,15,20-tetrakis (4-carboxylphenyl) porphyrin functionalized NiCo2S4 yolk-shell nanospheres: Excellent peroxidase-like activity, catalytic mechanism and fast cascade colorimetric biosensor for cholesterol. SENSORS AND ACTUATORS B-CHEMICAL, [10.1016/j.snb.2020.128850]
5. Wang Nan, Sun Jianchao, Chen Lijian, Fan Hai, Ai Shiyun. (2015) A Cu2(OH)3Cl-CeO2 nanocomposite with peroxidase-like activity, and its application to the determination of hydrogen peroxide, glucose and cholesterol. MICROCHIMICA ACTA, 182 (9): (1733-1738). [10.1007/s00604-015-1506-8]
6. Chaochao Shen, Xin Xi, Dongqing Wu, Xiaojun Guo, Yuezeng Su, Ruili Liu. (2024) Active-matrix extended-gate field-effect transistor array for simultaneous detection of multiple metabolites. BIOSENSORS & BIOELECTRONICS, [PMID:39305822] [10.1016/j.bios.2024.116787]
7. Ziyi Tong, Shengyan Hou, Zhenkun Zhang, Zhen Liu, Yifei Zhang. (2024) Biochemical approaches for decoding the information stored with metabolites. SENSORS AND ACTUATORS B-CHEMICAL, [10.1016/j.snb.2024.136618]
8. Shuangqian Yan, Panpan Xue, Ying Sun, Tingjie Bai, Sijie Shao, Xuemei Zeng. (2024) Cupric Doping Hollow Prussian Blue Nanoplatform for Enhanced Cholesterol Depletion: a Promising Strategy for Breast Cancer Therapy and Metastasis Inhibition. Advanced Science, [PMID:39606805] [10.1002/advs.202409967]
9. Pang Tingyuan, Liu Xiaorui, Li Jia, Song Qi, Li Junren, Han Ling, Shu Wenying, Jian Xiaoshun, Zhang Meimei. (2024) Highly sensitive and accurate detection of cholesterol based on a single red upconversion biosensor. RSC Advances, 14 (11): (7858-7866). [PMID:38449817] [10.1039/D3RA07354H]
10. Guo Qi, Wang Du, Ma Fei, Fang Mengxue, Zhang Liangxiao, Li Peiwu, Yu Li. (2024) MOF-derived nanozyme CuOx@C and its application for cascade colorimetric detection of phytosterols. MICROCHIMICA ACTA, 191 (6): (1-14). [PMID:38717599] [10.1007/s00604-024-06389-y]
11. Yuanyuan Zhang, Lexian wu, Jing Yang, Guoming Li, Keqin Deng, Haowen Huang. (2024) Platinum Glutamate Acid Complex as a Peroxidase Mimic: High Activity, Controllable Chemical Modification, and Application in Biosensor. Analytical Methods, [PMID:38284245] [10.1039/D3AY02121A]
12. Meiling Chen, Yang Yang, Qinhua Chen, Lina Tang, Junlin Liu, Yujie Sun, Qiming Liu, Yulin Zhang, Guo-jun Zhang, Shaowei Chen. (2024) Pt,P-codoped carbon nitride nanoenzymes for fluorescence and colorimetric dual-mode detection of cholesterol. ANALYTICA CHIMICA ACTA, [PMID:38438235] [10.1016/j.aca.2024.342351]
13. Xiangling Li, Shantao Zheng, Mengyi He, Xinshuo Huang, Cheng Yang, Jingshan Mo, Jingbo Yang, Chengduan Yang, Huijiuan Chen, Xi Xie. (2025) Self-calibrating multiplexed microneedle electrode array for continuous mapping of subcutaneous multi-analytes in diabetes. The Innovation, [PMID:39991476] [10.1016/j.xinn.2024.100781]
14. Xinying Ma, Zhe Zhang, Yanyan Zheng, Jiyang Liu. (2024) Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol. Biosensors-Basel, 14 (8): (403). [PMID:39194632] [10.3390/bios14080403]
15. Yanmei Lu, Jiayue Liu, Yufang Hu, Wenjun Dong, Caizhao Cheng, Zhihe Qing, Shun Zhang. (2024) Visual and quantitative determination of KAT Tip60 activity in circulating tumor cells using a smartphone. SENSORS AND ACTUATORS B-CHEMICAL, [10.1016/j.snb.2024.135827]
16. Xuemei Zeng, Shumin Tang, Guosheng Hu, Yonghong Pang, Sihuang Lin, Shijie Shangguan, Shuangqian Yan, Xinglin Ruan, Liling Zheng. (2025) Reprogramming Macrophage Function via Cholesterol Modulation and Redox Signaling Using a Multifunctional Nanoplatform for Postoperative Breast Cancer Management. ACS Nano, [PMID:40905820] [10.1021/acsnano.5c08767]
17. Panpan Xue, Tingjie Bai, Huilan Zhuang, Angelo H. All, Shuangqian Yan, Xuemei Zeng. (2025) Cholesterol Metabolism Regulated Nanoliposome Ameliorates Chemo/Photothermal Therapy Reversing CD8+ T Cell Exhaustion. Exploration, [10.1002/EXP.20240123]
18. Hongrui Zhang, Ya-nan Zhang, Like Li, Xuegang Li, Yong Zhao. (2025) An Enzyme-Catalyzed Optical Fiber Optofluidic Laser Sensor for Cholesterol Detection Using Rhodamine B Hydrazide. ANALYTICAL CHEMISTRY, [PMID:39982367] [10.1021/acs.analchem.4c06462]
19. Kai Yan, Chonglun Wang, Ye Zhang, Yunfei Li, Ying Jin, Jingjing Jiang, Shengda Liu, Jiayin Zhan, Wensheng Yu, Xiangting Dong, Guixia Liu. (2025) Protein-stabilized gold nanoclusters for colorimetric/fluorescence dual-mode detection of organophosphorus pesticides. FOOD CHEMISTRY, [PMID:41259851] [10.1016/j.foodchem.2025.147115]
20. Chen Hu, Niu Feng, Nana Gao, Nan Liu, Shuailong Zhang, Aiqin Luo, Yiping Chen, Jiangjiang Zhang. (2026) Surface Passivation and Aggregation-Enhanced Emission of Luminescent Copper Nanoparticles for Advanced Biochemical Sensors. ANALYTICAL CHEMISTRY, [PMID:41779429] [10.1021/acs.analchem.5c06608]