来自马心脏的细胞色素c已被用于制备合成和标定生物素化细胞色素 c的储备溶液。 它还被用作反应缓冲液的成分,该缓冲液可用于染色电子传输链(ETC)复合物IV及用蓝色非变性凝胶电泳确定该复合物活性。马的细胞色素c经过H2O2和UV 处理后的特异性位点和氧化程度已经过表征。研究表明这些位点可能可用作细胞内氧化环境的生物标记。应用:
细胞色素C(Cyt C)是一种核DNA编码蛋白。它来源于它的前体脱血红素细胞色素 c,并可以穿过线粒体外膜。研究领域:细胞凋亡
Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria.
Research area: Apoptosis
Application:
Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis.
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.