荧光素是一种常用的生物发光报告分子，用于体内监测荧光素酶的表达。这种水溶性的萤火虫荧光素酶底物利用ATP和Mg²⁺作为辅助因子，在氧气存在时发出特征性黄绿色光，而在37°C的体内环境中会转变为红光。通过消耗ATP的特性，该反应还可作为能量或生命存在的指示剂，发挥生死指示功能。
荧光素作为生物技术领域的通用试剂，尤其适用于体内成像。当将荧光素酶标记的肿瘤细胞、干细胞或病原体接种至大鼠、小鼠等实验动物后，注射荧光素即可通过生物发光成像（BLI）技术，实现对疾病进程和药物疗效的实时、无创监测。
荧光素也通常用于体外研究，包括荧光素酶和ATP分析、报告基因分析、高通量测序和多种污染检测实验。
应用：
体内细胞成像
荧光素酶与ATP检测
报告基因分析
高通量测序
污染检测
检测缓冲液：
| TMCA缓冲液 | 终浓度
|
| Tris-HCl, pH 7.8 | 100mM
|
MgCl₂
| 5mM |
辅酶A (CoA) 水合物
| 0.25mM |
| ATP (二钠盐水合物) | 0.15mM |
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg²⁺ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).
Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.
APPLICATIONS
in vivo cellular imaging
luciferase and ATP assays
gene reporter assays
high throughput sequencing
contamination assays
Assay Buffer
| Tris-MgCl₂-CoA Buffer | Final Concentration |
| Tris-HCl, pH 7.8 | 100mM
|
MgCl₂
| 5mM |
| Coenzyme A (CoA) (hydrate) | 0.25mM |
| ATP (disodium salt hydrate) | 0.15mM |
ZJ26F0939315
危险品化学品经营许可证（带存储）