荧光素是一种常用的生物发光报告分子，用于荧光素酶表达的体内成像。这种萤火虫荧光素酶的底物以 ATP 和 Mg²⁺为辅因子，在有氧条件下发出特征性的黄绿色光，而在体内 37°C 环境下会转变为红光。由于该反应利用 ATP，因此还可通过检测其发光来指示能量或生命的存在，从而用作区分生死的染色剂。
荧光素是生物技术领域广泛使用的常规试剂，尤其适用于体内成像。研究中，常将荧光素酶标记的肿瘤细胞、干细胞或感染性疾病病原体接种到大鼠、小鼠等实验动物体内。通过注射荧光素，借助生物发光成像（BLI）技术，能够在这些模型系统中对疾病进展和 / 或药物疗效进行实时、无创的监测。
荧光素也常用于体外研究，包括荧光素酶和 ATP 检测、基因报告基因检测、高通量测序以及各种污染检测。
应用
体内细胞成像
荧光素酶和 ATP 检测
基因报告基因检测
高通量测序
污染检测
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This substrate for the firefly luciferase enzyme utilizes ATP and Mg²⁺ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).
Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.
APPLICATIONS
in vivo cellular imaging
luciferase and ATP assays
gene reporter assays
high throughput sequencing
contamination assays
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