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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
CD68分子(CD68)是一种由单核细胞系的细胞(如单核吞噬细胞、破骨细胞)、循环巨噬细胞和组织巨噬细胞(如Kupffer细胞、小胶质细胞)高度表达的蛋白质。CD68在功能和进化上与其他基因/蛋白家族成员有关:造血粘蛋白类分子家族,包括白血球蛋白/CD43和干细胞抗原CD34;溶酶体/内体相关膜糖蛋白(LAMP)家族,它主要定位在溶酶体和内体,但有一小部分循环到细胞表面;清道夫受体家族,其典型功能是清除细胞碎片,促进吞噬作用,并介导巨噬细胞的招募和激活。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠CD68分子(CD68)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠CD68分子(CD68)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse CD68. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse CD68 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.