计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尿激酶型纤溶酶原激活物受体(uPAR),也被称为尿激酶受体或PLAUR或CD87,是由PLAUR基因编码的蛋白质。它是一种多结构域糖蛋白,通过糖磷脂酰肌醇锚定在细胞膜上。它最初被鉴定为细胞表面尿激酶(也称为uPA)的可饱和结合位点。它在多种癌细胞表明都有发现。它是纤溶酶原激活系统的一部分,它在健康体内参与组织重组事件,例如乳腺退化和伤口愈合。为了能够重组组织,旧组织必须能够降解。这种降解的一个重要机制是由纤溶酶原激活系统引发的蛋白水解级联反应。它结合尿激酶,从而将纤溶酶原激活限制在细胞膜附近。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠尿激酶型纤溶酶原启动因子受体(uPAR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠尿激酶型纤溶酶原启动因子受体(uPAR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse uPAR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse uPAR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.