计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
信号传导转录激活因子2是一种蛋白质,由STAT2基因编码。它是STAT蛋白家族的成员。该蛋白对I型干扰素(IFN)的生物反应至关重要。小鼠和人类之间的STAT2序列一致性只有68%。该基因所编码的蛋白是STAT蛋白家族的成员。在对细胞因子和生长因子的反应中,STAT家族成员被受体相关激酶磷酸化,然后形成同源或异源二聚体,转入细胞核,在那里充当转录激活剂。在对IFN的反应中,该蛋白与STAT1和IFN调节因子家族蛋白p48(IRF9)形成复合物,并形成ISGF-3(IFN刺激的基因因子-3),该蛋白作为转录激活剂,但缺乏直接结合DNA的能力。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人信号传导转录激活因子2(STAT2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人信号传导转录激活因子2(STAT2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human STAT2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human STAT2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.