BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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可溶性尿激酶型纤溶酶原激活物受体(suPAR)是一种蛋白质，是uPAR的可溶性形式。它是在炎症或免疫激活期间裂解和膜结合的uPAR的结果。它的浓度与免疫系统的激活水平呈正相关。它是疾病严重性和侵略性的标志，与一些急性和慢性疾病的发病率和死亡率有关。已经观察到suPAR水平随着年龄的增长而增加。suPAR 水平与促炎症生物标志物正相关，包括肿瘤坏死因子-α（TNF-α）和 C 反应蛋白（CRP）以及其他参数，包括白细胞计数。它也与各种疾病的器官损伤有关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人可溶性尿激酶型纤溶酶原激活物受体(suPAR)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人可溶性尿激酶型纤溶酶原激活物受体(suPAR)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human suPAR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human suPAR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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