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尿激酶型纤溶酶原激活物受体(uPAR),又称尿激酶受体或CD87(Cluster of Differentiation 87),是人类由PLAUR基因编码的一种蛋白质。它是一个多域糖蛋白,用一个糖基磷酰肌醇(GPI)锚拴在细胞膜上。uPAR最初被确定为细胞表面尿激酶(也称为uPA)的可饱和结合点。uPAR由三个串联的LU结构域组成,是三指蛋白家族的蛋白结构域。uPAR与肽拮抗剂和其本地配体尿激酶复合物的结构已通过X射线晶体学得到解决。此外,uPAR还与其他几种蛋白相互作用,包括维管蛋白、uPAR相关蛋白(uPARAP)和膜蛋白的整合素家族。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人尿激酶型纤溶酶原激活物受体(uPAR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人尿激酶型纤溶酶原激活物受体(uPAR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human uPAR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human uPAR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.