双-PEG7-酸：性质、操作与应用指南
Bis-PEG7-acid的全面指南：结构、溶解性、储存条件、生物偶联方案及PROTAC接头应用，专为科研人员设计。
主题
PROTAC
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
18 项资源 · 实验方案、常见问题与文章
Bis-PEG7-acid: Properties, Handling & Application Guide
Comprehensive guideline on Bis-PEG7-acid: structure, solubility, storage, bioconjugation protocols, and PROTAC linker applications for research scientists.
酸-PEG5-叔丁酯：操作与应用指南
逐步指导Acid-PEG5-叔丁酯（A596986）的储存、操作及在生物偶联与PROTAC研究中作为PEG连接子的使用方法。
Acid-PEG5-t-butyl ester: Handling & Application Guidelines
Step-by-step guidelines for storing, handling, and using Acid-PEG5-t-butyl ester (A596986) as a PEG linker in bioconjugation and PROTAC research.
氨基-PEG36-CONH-PEG36-酸：长链双功能PEG连接子
探索Amino-PEG36-CONH-PEG36-acid（A596370），一种用于生物偶联、PROTAC及药物递送研究的长链双功能PEG连接体。
Amino-PEG36-CONH-PEG36-acid: Long-Chain Bifunctional PEG Linker
Explore Amino-PEG36-CONH-PEG36-acid (A596370), a long-chain bifunctional PEG linker for bioconjugation, PROTAC, and drug delivery research.
3-(4-Piperazin-1-ylanilino)piperidine-2,6-dione Dihydrochloride Guide
Handling, storage, and research-use guidelines for 3-(4-Piperazin-1-ylanilino)piperidine-2,6-dione dihydrochloride (SKU P1529534) from Aladdin Scientific.
3-(4-哌嗪-1-基苯胺基)哌啶-2,6-二酮二盐酸盐指南
Aladdin Scientific提供的3-(4-哌嗪-1-基苯胺基)哌啶-2,6-二酮盐酸盐（货号P1529534）的操作、储存及科研使用指南。
KRAS G12D：如何从直接抑制走向靶向降解
KRAS G12D是胰腺癌、结直肠癌和肺癌等实体瘤中的重要驱动突变。MRTX1133等分子的出现证明了KRAS G12D可以被直接药理干预，而PROTAC等靶向蛋白降解技术进一步将作用模式从“占据并抑制KRAS”推进到“募集E3连接酶并清除KRAS蛋白”。
KRAS G12D: How Direct Inhibition Is Advancing Toward Targeted Degradation
KRAS G12D is an important driver mutation in solid tumors such as pancreatic, colorectal, and lung cancers. The emergence of molecules such as MRTX1133 has demonstrated that KRAS G12D can be directly targeted pharmacologically, whereas targeted protein degradation technologies such as PROTACs ...
从PROTAC到非泛素-蛋白酶体降解：LYTAC、ATTEC、AUTAC与BacPROTAC的机制与选择
经典PROTAC依赖E3泛素连接酶和蛋白酶体清除胞内靶蛋白，但难以覆盖胞外蛋白、大尺度细胞组分及细菌蛋白。LYTAC、ATTEC、AUTAC和BacPROTAC分别利用内吞-溶酶体、自噬-溶酶体或原核蛋白酶系统，为不同空间位置和物理尺度的靶标建立定向清除途径。 ...
From PROTAC to Non-Ubiquitin–Proteasome Degradation: Mechanisms and Selection of LYTAC, ATTEC, AUTAC, and BacPROTAC
Classical PROTACs rely on E3 ubiquitin ligases and the proteasome to eliminate intracellular target proteins, but they are difficult to apply to extracellular proteins, large-scale cellular components, and bacterial proteins. LYTAC, ATTEC, AUTAC, and BacPROTAC respectively utilize ...
New Directions in RAS-Targeted Therapy: From Active-Conformation Inhibition to Protein Degradation
RAS mutations drive many tumors, including pancreatic cancer, colorectal cancer, and lung cancer. However, RAS was long considered difficult to drug because of its shallow structural pockets and tight GTP binding. The emergence of RAS(ON) inhibitors, molecular glues, and PROTACs has shifted RAS ...
RAS靶向治疗新方向：从活性构象抑制到蛋白降解
RAS突变驱动胰腺癌、结直肠癌和肺癌等多种肿瘤，但因结构口袋浅、GTP结合紧密，长期难以成药。RAS(ON)抑制剂、分子胶和PROTAC的出现，使RAS干预从阻断信号走向清除致癌蛋白。
Three Key Components of PROTAC Design: E3 Ligase Ligands, Linkers, and Target Protein Ligands (with an Aladdin Reagent Selection Guide)
PROTACs (PROteolysis-TArgeting Chimeras) are heterobifunctional small molecules that induce targeted protein degradation. Each PROTAC molecule contains two distinct ligands: one is a target protein ligand that binds specifically to the intracellular protein of interest (POI), and the other is a ...
PROTAC设计三要素：E3连接酶配体、Linker与靶蛋白配体（附阿拉丁试剂选型指南）
PROTAC（PROteolysis-TArgeting Chimeras，蛋白降解靶向嵌合分子）是一类杂合双功能小分子化合物，其结构中包含两种不同配体：一端为与细胞内目标蛋白（Protein of Interest, ...
Targeted protein degradation technology: proprietary drugs are not difficult, drug resistance is no longer
The anticancer drug Lenalidomide was once one of the world's top-selling small molecule drugs and orphan drugs, with sales of up to 9.978 billion US dollars in 2022, and its development company Celgene made huge profits.
靶向蛋白降解技术: 成药不难，耐药不再
抗癌药物来那度胺（Lenalidomide）曾是世界上销量最高的小分子药物和孤儿药之一，在2022年的销售额高达99.78亿美元，其开发公司Celgene获得了巨额利润。
相关主题
常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）