计算溶液所需的质量、体积或浓度。
改良苏木素-伊红(HE)染色试剂盒
有货
为什么选择此级别
BioReagent,用于显微镜,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
改良苏木素 - 伊红（HE）染色试剂盒是病理切片常规染色的核心工具，操作便捷、染色效果稳定，广泛用于组织形态学观察，主要用于生物组织切片（如石蜡切片、冰冻切片）的形态学染色，能清晰区分细胞核与细胞质，帮助观察组织结构、细胞形态及病理变化，是病理诊断、科研实验中最基础的染色方法。
| 项目号 | Components | 100T | Storage |
| H1506002A | 苏木素染液 | 100 mL | RT. Store in the dark. |
| H1506002B | 伊红染液 | 100 mL | RT |
| H1506002C | 反蓝试剂 | 100 mL | RT |
| H1506002D | 分化液 | 100 mL | RT |
自备材料：
1、1%酸性乙醇分化液,亦可采购酸性乙醇分化液
2、弱碱性水，亦可采购氨水溶液(0.1%)
3、碳酸锂水溶液,亦可采购碳酸锂水溶液(1%)
4、无水乙醇、95%乙醇、90%乙醇、80%乙醇等系列乙醇
5、4%的多聚甲醛，亦可采购多聚甲醛溶液(4%PFA)
操作步骤（仅供参考）：
石蜡切片染色：
1、切片脱蜡至水
①二甲苯作用2次，每次5～10min。
②（可选）无水乙醇作用2次，每次3～5min。
③95%的乙醇3～5min
④90%的乙醇3～5min
⑤80%的乙醇3～5min
⑥自来水或蒸馏水冲洗1～3min
2、染色
①苏木素染色液染色5～8min
②蒸馏水冲洗5～10s，洗去多余的染色液
③切片经分化液2～5s，自来水流水充分冲洗
④返蓝液返蓝2～5s，自来水流水充分冲洗
⑤伊红染色液染色3～5min
3、脱水、透明、封固
①80%乙醇10～20s
②90%乙醇10～20s
③95%乙醇作用2次，每次1～2min。
④无水乙醇作用2次，每次2～3min。
⑤二甲苯透明3次，每次2～3min。
⑥中性树脂封片。
染色结果：细胞核呈蓝色；细胞质、肌纤维、胶原纤维、甲状腺胶质等呈深浅不一的红色；角蛋白、红细胞等呈明亮的橙红色。
冰冻切片染色：
①丙酮或多聚甲醛固定5～10s
②自来水冲洗2～5s
③苏木素染色液滴染1～2min(可加热至50°C)
④自来水冲洗2～5s
⑤切片经分化液2～5s，自来水流水充分冲洗
⑥返蓝液返蓝2～5s，自来水流水充分冲洗
⑦伊红染色液染色2～5s
⑧水洗1～2s
⑨80%的乙醇1～2s
⑩95%的乙醇1～2s
⑪无水乙醇2～5s
⑫苯酚二甲苯（1:3）2～5s
⑬二甲苯透明3次，每次2～5s
⑭中性树脂封片
注意事项：
1.切片脱蜡应尽量干净。
2.95%乙醇、90%乙醇、80%乙醇应经常更换新液。
3.1%的酸性乙醇分化液的分化时间应该依据切片厚薄、组织的类别和1%的盐酸乙醇分化液的新旧而定，另外分化后自来水冲洗时间应该足够，以便彻底清洗酸。
4. 乙醚-乙醇混合固定液是由乙醚和95%乙醇等量混合而得，再加入适量乙酸，密闭保存。
5.冷冻切片染色时间尽量要短。
6.促蓝液常使用0.2～1%氨水水溶液或Scoot促蓝液或0.1～1%碳酸锂水溶液。
7、为了您的安全和健康，请穿实验服并戴一次性手套操作。
The Modified Hematoxylin-Eosin (HE) Staining Kit is a core tool for routine staining of pathological sections. It features convenient operation and stable staining effects, and is widely used in histological morphology observation. Primarily designed for morphological staining of biological tissue sections (such as paraffin sections and frozen sections), it can clearly distinguish cell nuclei from cytoplasm, aiding in the observation of tissue structure, cell morphology, and pathological changes. It serves as the most fundamental staining method in pathological diagnosis and scientific research experiments.
| H1506002 | Components | 100T | Storage |
| H1506002A | Hematoxylin Staining | 100 mL | RT. Store in the dark. |
| H1506002B | Eosin Staining | 100 mL | RT |
| H1506002C | Blueing Reagent | 100 mL | RT |
| H1506002D | Differentiating Solution | 100 mL | RT |
Self-Prepared Materials:
1. 1% acidic ethanol differentiating solution; alternatively, acidic ethanol differentiating solution can be purchased.
2. Weakly alkaline water; alternatively, ammonia solution (0.1%) can be purchased.
3. Lithium carbonate aqueous solution; alternatively, lithium carbonate aqueous solution (1%) can be purchased.
4. A series of ethanol solutions, including absolute ethanol, 95% ethanol, 90% ethanol, and 80% ethanol.
5. 4% paraformaldehyde; alternatively, paraformaldehyde solution (4% PFA) can be purchased.
Operating Procedures (For Reference Only):
Staining of Paraffin Sections:
1. Dewaxing of Sections to Water
① Treat with xylene twice, 5–10 minutes each time.
② (Optional) Treat with absolute ethanol twice, 3–5 minutes each time.
③ Treat with 95% ethanol for 3–5 minutes.
④ Treat with 90% ethanol for 3–5 minutes.
⑤ Treat with 80% ethanol for 3–5 minutes.
⑥ Rinse with tap water or distilled water for 1–3 minutes.
2. Staining
① Stain with hematoxylin staining solution for 5–8 minutes.
② Rinse with distilled water for 5–10 seconds to remove excess staining solution.
③ Immerse the sections in differentiation solution for 2–5 seconds, then rinse thoroughly with running tap water.
④ Treat the sections with bluing solution for 2–5 seconds, then rinse thoroughly with running tap water.
⑤ Stain with eosin staining solution for 3–5 minutes.
3. Dehydration, Clearing, and Mounting
① Treat with 80% ethanol for 10–20 seconds.
② Treat with 90% ethanol for 10–20 seconds.
③ Treat with 95% ethanol twice, 1–2 minutes each time.
④ Treat with absolute ethanol twice, 2–3 minutes each time.
⑤ Clear with xylene three times, 2–3 minutes each time.
⑥ Mount sections with neutral resin.
Staining Results
Cell nuclei: blue.
Cytoplasm, muscle fibers, collagen fibers, and thyroid colloid: red of varying intensities.
Keratin and red blood cells: bright orange-red.
Staining of Frozen Sections
① Fix with acetone or paraformaldehyde for 5–10 seconds.
② Rinse with tap water for 2–5 seconds.
③ Drop-stain with hematoxylin staining solution for 1–2 minutes (may be heated to 50°C).
④ Rinse with tap water for 2–5 seconds.
⑤ Immerse the sections in differentiation solution for 2–5 seconds, then rinse thoroughly with running tap water.
⑥ Treat the sections with bluing solution for 2–5 seconds, then rinse thoroughly with running tap water.
⑦ Stain with eosin staining solution for 2–5 seconds.
⑧ Rinse with water for 1–2 seconds.
⑨ Treat with 80% ethanol for 1–2 seconds.
⑩ Treat with 95% ethanol for 1–2 seconds.
⑪ Treat with absolute ethanol for 2–5 seconds.
⑫ Treat with phenol-xylene (1:3, v/v) for 2–5 seconds.
⑬ Clear with xylene for 3 times, 2–5 seconds each time.
⑭ Mount the sections with neutral balsam.
Precautions:
1. Paraffin sections should be thoroughly deparaffinized.
2. The 95% ethanol, 90% ethanol, and 80% ethanol solutions should be replaced regularly with fresh solutions.
3. The differentiation time in 1% acid-alcohol differentiation solution should be determined based on the thickness of the sections, the type of tissue, and the freshness of the 1% acid-alcohol differentiation solution. Additionally, after differentiation, the sections should be rinsed sufficiently with running water to completely remove the acid.
4. The ether-ethanol fixation solution is prepared by mixing equal amounts of ether and 95% ethanol, followed by adding an appropriate amount of acetic acid. It should be stored in a sealed container.
5. The staining time for frozen sections should be as short as possible.
6. Bluing solutions commonly used include 0.2–1% ammonia water, Scoot bluing solution, or 0.1–1% lithium carbonate aqueous solution.
7. For your safety and health, please wear a lab coat and disposable gloves during operations.
规格
别名
苏木素-伊红染色试剂盒
英文别名
HE Staining Kit | Hematoxylin-Eosin Staining Kit
规格或纯度
BioReagent,用于细胞培养,用于显微镜
英文名称
Modified Hematoxylin-Eosin (HE) Staining Kit
应用
细胞染色
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
Store at RT long term (12 months). Store in the dark.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0737653 |
分析证书 | H1506002 |
| ZJ26F0131347 |
分析证书 | H1506002 |
技术文档和文章
Applications and Comparison of Histological Counterstaining, Collagen Evaluation, and Protein Staining Methods
目录： 技术文章
Eosinophil Morphological Observation: Staining Basis, Identification Points, and Research Validation
目录： 技术文章
Microscopic Detection of Blood Parasites: Thick and Thin Blood Film Preparation and Romanowsky-Type Staining Systems
目录： 技术文章
线虫与虫卵形态学观察方法
目录： 技术文章
溶液计算器
常见问题
什么是「用于显微镜」？
「用于显微镜」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
该抗体已验证对哪些物种有反应性？
已验证的反应性列在PDP和每批次的数据表上。我们针对靶标（Modified Hematoxylin-Eosin (HE) Staining Kit）最常测试的物种进行验证；未经测试的物种可能存在交叉反应性，但不能保证。如有特定物种咨询，请联系技术支持。
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