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3-甲基腺嘌呤（3-MA）是一种已被用作DNA甲基化体内标志物的腺嘌呤甲基化衍生物。3-甲基腺嘌呤和7-甲基鸟嘌呤是DNA暴露于甲基化剂后形成的两种主要的N-甲基嘌呤。3-MA可通过DNA碱基切除修复途径而从受损的DNA中被去除，而该途径始于N-甲基嘌呤DNA糖基化酶。3-MA修复对哺乳动物细胞系中核苷酸切除修复的依赖性已得到了研究。3-MA因其对I类和III类PI3K活性的抑制作用而被广泛用作自噬抑制剂。 且已知这对于自噬的诱导是必需的。3-MA可持续阻断I类PI3K而其对
应用
3-甲基腺嘌呤（3-MA）用于抑制和研究多种条件下自噬（溶酶体自我降解）和细胞凋亡的机制。3-MA可通过抑制III型磷脂酰肌醇3-激酶（PI-3K）阻断自噬体的形成来对自噬进行抑制。3-MA通常以5 mM的浓度用作自噬抑制剂。
适用性
原理
制备说明
3-甲基腺嘌呤即用型溶液是在MES缓冲液中以50mM（7.5mg/mL）的浓度提供的。建议将3-甲基腺嘌呤即用型溶液储存在-20C。如果融化时形成沉淀，可使用超声处理和加热而使其重新溶解。建议避免反复冻融。正确储存的3-甲基腺嘌呤即用型溶液应当可以稳定2年。
外形
3-Methyladenine (3-MA) is a methylated derivative of adenine that has been used as anIn vivomarker for the methylation of DNA. 3-Methyladenine and 7-methylguanine are the two main N-methylpurines formed in DNA upon exposure to methylating agents. 3-MA is removed from damaged DNA via the DNA base excision repair pathway, which begins with the enzyme N-methylpurine DNA glycosylase. The dependence of 3-MA repair on nucleotide excision repair in mammalian cell lines has been studied.3-MA have been widely used as autophagy inhibitor based on its inhibitory effect on class I and class III PI3K activity, which is known to be essential for induction of autophagy. 3-MA blocks class I PI3K persistently, whereas its suppressive effect on class III PI3K is transient, understanding the dual role of 3-MA in autophagy thus suggests that caution should be exercised in the application of 3-MA in autophagy study.Furthermore, 3-MA is also capable of making the reduction of lymphocyte number caused by chronic stress rebound, implying that inhibition of autophagy can rescue chronic stress-induced immunosuppression.Autophagy has been reported to play an important role in the 3, 4-Methylenedioxymethamphetamine MDMA-induced neurotoxicity in the mixed neuronal-glial primary culture and it was found that 3-MA ameliorate MDMA-induced autophagy, SERT loss, HPA axis dysfunction, and depressive behaviors.3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5mM.
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