本品为来源于健康人血浆的天然血纤维蛋白溶酶原,正常人血浆中含量约为12 mg/dL,作为丝氨酸蛋白酶纤溶酶的前体,在纤维蛋白溶解过程中发挥关键作用,其消光系数(E₁%¹cm)为1.71。所用原料血浆均经严格筛查,并按照美国FDA相关规定进行检测,乙肝表面抗原(HBsAg)、抗‑HCV抗体、抗‑HBc抗体及抗‑HIV‑1/2抗体均为阴性,检测记录可溯源备查。
产品活性采用TAME(N‑对甲苯磺酰‑L‑精氨酸甲酯)酯水解法测定:纤溶酶原经链激酶激活后,于0.1 mol/L Tris缓冲液(pH 8.0)中与TAME底物在37 °C孵育15分钟,于510 nm波长处测定吸光度,经线性拟合计算比活力(U/mg)。
Found at 12 mg per 100 ml normal plasma. It is the precursor of the serine protease plasmin which s involved in fibrinolysis.
Extinction Coefficient: 1.71
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
The activity of this product is evaluated using a TAME (N-tosyl-L-arginine methyl ester) esterolytic activity assay. Briefly, plasminogen is activated with streptokinase and incubated in 0.1 M Tris buffer (pH 8.0) with the TAME substrate. The reaction mixture is incubated at 37 °C for 15 minutes, and absorbance is measured at 510 nm using a spectrophotometer. The resulting values are plotted linearly to determine the specific activity (U/mg).
One unit of activity is defined as the amount of enzyme that hydrolyzes 1 µmole of TAME substrate in 30 minutes at 37 °C, pH 8.0.