聚乙烯醇甘油封片剂 (Elvanol Glycerol Mounting Medium)属于抗荧光衰减封片剂 (Fluorescence decay resistant Medium)的一种，是一种用于减缓荧光淬灭的封固剂。可以用于减缓各种常见荧光染料的荧光淬灭，较普通抗荧光淬灭封片剂的抗淬灭效果大大增强，一般1-2周后才会有所减弱，按每个样本需要50-100μl封片剂计算，5ml可以用于至少50样本的封片。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、载玻片
2、盖玻片
3、荧光显微镜
操作步骤(仅供参考)：
1、贴壁细胞样本
①染色完成后，吸去染色液。
②滴1滴聚乙烯醇甘油封片剂于载玻片上，盖上贴有细胞的盖玻片，让细胞接触封片液，尽量避免气泡。
③在荧光显微镜下观察或4℃避光保存1-2周。
2、组织切片
①染色完成后，吸去染色液。
②滴1滴聚乙烯醇甘油封片剂于组织切片上，盖上盖玻片，让切片接触封片液，尽量避免气泡。
③在荧光显微镜下观察或4℃避光保存1-2周。
3、其它样品
其它样品参考细胞样品或组织切片进行操作。
注意事项：
1、抗荧光淬灭的概念是相对的，样本染色后最好立即观察，如果没有条件，最好应在当天观察。
2、荧光物质均易发生淬灭，染色后的样品宜4℃避光保存。
3、在使用聚乙烯醇甘油封片剂的情况下，虽然可减缓淬灭，仍需尽量避光。
4、用罗丹明标记的抗体不能用该封片剂封片，可以采用聚乙烯醇荧光封片剂。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Product Introduction
Polyvinyl Alcohol-Glycerol Mounting Medium is a type of Fluorescence Decay-Resistant Mounting Medium, a mounting agent designed to slow down fluorescence quenching. It can reduce the quenching effect of various common fluorescent dyes, with its anti-quenching performance significantly superior to that of regular anti-quenching mounting media. Generally, the anti-quenching effect only starts to diminish after 1–2 weeks. Based on a dosage of 50-100 μl per sample, 5 ml of the reagent can be used for mounting at least 50 samples. This reagent is intended for research purposes only and not for clinical diagnosis or other applications.
Materials to Be Prepared by Users
1. Slides
2. Coverslips
3. Fluorescence microscope
Operating Procedures (For Reference Only)
1. Adherent Cell Samples
① After staining, aspirate the staining solution completely.
② Add 1 drop of Polyvinyl Alcohol-Glycerol Mounting Medium onto the slide, then place the coverslip with adherent cells onto the medium. Ensure the cells are in contact with the mounting medium and minimize bubble formation.
③ Observe under a fluorescence microscope immediately, or store the slides at 4 °C away from light for 1-2 weeks.
2. Tissue Sections
① After staining, aspirate the staining solution completely.
② Add 1 drop of Polyvinyl Alcohol-Glycerol Mounting Medium onto the tissue section, then place a coverslip over it. Ensure the section is in contact with the mounting medium and minimize bubble formation.
③ Observe under a fluorescence microscope immediately, or store the slides at 4 °C away from light for 1-2 weeks.
3. Other Samples
Follow the procedures for adherent cell samples or tissue sections.
Precautions
1. The concept of anti-fluorescence quenching is relative. Stained samples are best observed immediately after staining; if immediate observation is not feasible, observation should be completed on the same day.
2. All fluorescent substances are prone to quenching. Stained samples should be stored at 4 °C away from light.
3. Although this Polyvinyl Alcohol-Glycerol Mounting Medium can slow down quenching, samples should still be protected from light as much as possible during use and storage.
4. Antibodies labeled with rhodamine cannot be mounted with this medium; Polyvinyl Alcohol Fluorescent Mounting Medium is recommended instead.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. Use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.
ZJ26F0232079
危险品化学品经营许可证（带存储）