BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丙酮酸(Pyruvic acid，PA)又称2-氧代丙酸，是参与整个生物体基本代谢的中间产物之一，可通过乙酰辅酶A和三羧酸循环实现体内糖、脂肪和氨基酸间的互相转化，丙酮酸在三大营养物质的代谢联系中起着重要的枢纽作用。丙酮酸和乳酸是糖无氧代谢的产物，科研工作者常将二者一起研究，并用二者的比值推算循环衰竭的程度，丙酮酸检测可采用乳酸脱氢酶催化法、二硝基苯肼法等，二硝基苯肼法是比较古老的方法，生成有色物质，易于观察，但易受α-酮酸的干扰，特异性差，操作烦琐。目前首选方法是乳酸脱氢酶催化法。 该试剂盒的检测原理是在NADH存在条件下，乳酸脱氢酶(LDH)催化丙酮酸氧化，生成乳酸和NAD⁺，在弱碱性条件下平衡偏向丙酮酸氧化为乳酸的方向驱动反应，通过酶标仪测定340nm处NADH吸光度的下降速率，计算出丙酮酸含量。可用于检测全血血浆样品中内源性的丙酮酸含量。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Pyruvic Acid (PA), also known as 2-oxopropanoic acid, is a crucial intermediate in fundamental metabolism across living organisms. It serves as a central metabolic hub, enabling the interconversion of carbohydrates, fats, and amino acids via acetyl-CoA and the tricarboxylic acid (TCA) cycle. Both pyruvate and lactate are end products of anaerobic glycolysis. Researchers often analyze them together, using their ratio to estimate the degree of circulatory failure. Methods for pyruvate detection include the lactate dehydrogenase (LDH) catalytic assay and the dinitrophenylhydrazine method. The latter is an older technique that relies on color formation for easy observation, but it suffers from interference by α-keto acids, poor specificity, and cumbersome procedures. Currently, the lactate dehydrogenase catalytic assay is the preferred method. The detection principle of this kit is based on the LDH-catalyzed oxidation of pyruvate to lactate in the presence of NADH, which generates NAD⁺. Under weakly alkaline conditions, the reaction equilibrium favors the oxidation of pyruvate to lactate. By measuring the rate of decrease in NADH absorbance at 340 nm using a microplate reader, the pyruvate concentration can be calculated. This kit is suitable for detecting endogenous pyruvate levels in whole blood plasma samples. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.
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