L2417280计算溶液所需的质量、体积或浓度。
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
|---|
有货
Accession #
P61823
为什么选择此级别
EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 37 篇同行评审文献引用。
概述
Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
规格
产品名称
核糖核酸酶A 来源于牛胰腺(纯化溶液)
英文别名
9001-99-4 | Nuclease, ribo- | L-HISTIDINE, N-beta-ALANYL- | 2-(3-aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid | 9001-73-4 | DL-(3-aminopropanoyl)histidine | 108333-82-0 | 2-(3-aminopropanamido)-3-(1H-imidazol-5-yl)propanoic acid | beta-Alanylhistidine | L-Histid
规格或纯度
EnzymoPure™, ≥3,000 units/mg protein
Accession #
CAS编号和信息
9001-99-4
分子类型
小分子
储存与运输
浓度
≥3,000 units/mg protein
储存条件
-20°C储存
运输条件
超低温运输
技术文档
高级数据
关联靶点（人）
ACHN
(49357 活性数据)
CAKI-1
(44928 活性数据)
CCRF-CEM
(65223 活性数据)
COLO 205
(50209 活性数据)
DU-145
(51482 活性数据)
HL-60
(67320 活性数据)
HT-29
(80576 活性数据)
K562
(73714 活性数据)
KM12
(47707 活性数据)
M14
(47487 活性数据)
MCF7
(126967 活性数据)
MOLT-4
(49676 活性数据)
OVCAR-3
(48710 活性数据)
OVCAR-4
(44535 活性数据)
OVCAR-5
(45555 活性数据)
OVCAR-8
(47708 活性数据)
PC-3
(62116 活性数据)
RPMI-8226
(44974 活性数据)
SF-295
(48000 活性数据)
SK-MEL-2
(46422 活性数据)
SK-MEL-28
(48833 活性数据)
SK-MEL-5
(47095 活性数据)
SN12C
(47755 活性数据)
SNB-19
(46794 活性数据)
TK-10
(45540 活性数据)
U-251
(51189 活性数据)
UACC-257
(46019 活性数据)
UO-31
(46270 活性数据)
786-0
(47912 活性数据)
A549
(127892 活性数据)
NCI/ADR-RES
(33767 活性数据)
T47D
(39041 活性数据)
EKVX
(44102 活性数据)
NCI-H322M
(45589 活性数据)
HCC 2998
(41480 活性数据)
HCT-116
(91556 活性数据)
HOP-92
(41141 活性数据)
Hs-578T
(29457 活性数据)
NCI-H460
(60772 活性数据)
SF-268
(49410 活性数据)
IGROV-1
(47897 活性数据)
LOX IMVI
(44321 活性数据)
HOP-62
(47048 活性数据)
MDA-MB-435
(38290 活性数据)
MDA-N
(28205 活性数据)
Malme-3M
(44254 活性数据)
MDA-MB-231
(73002 活性数据)
SNB-75
(44215 活性数据)
NCI-H226
(44470 活性数据)
NCI-H23
(49055 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
蛋白酶K与核糖核酸酶A
目录： 技术文章
质粒的五个关键结构特征
目录： 技术文章
Interleukin-29 (IL-29/IFN-λ1): Molecular Features, Signaling Networks, and a Systematic Framework for Research Applications
目录： 技术文章
dsDNase-Mediated Hydrolysis of Double-Stranded DNA: Enzymatic Characteristics, Methodological Strategies, and Bioanalytical Applications
目录： 技术文章
Physicochemical Properties of Diphenylamine, Analytical Applications, and a Review of Its DNA Colorimetric Reaction
目录： 技术文章
A Functional Systems Review of Cellular Nucleic Acid Metabolism and Regulatory Enzymes in Gene Expression, RNA Homeostasis, and Immune Defense
目录： 技术文章
Structural Characteristics, Operational Methods, and Separation Applications of Dextran Gels
目录： 技术文章
此产品的引用文献
引用文献
| 1. Ningning Lei, Xu Peng, Mengyue Hu, Chang Wan, Xixun Yu. (2023) Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:37100332] [10.1016/j.ijbiomac.2023.124522] |
| 2. Qing Liu, Chao Yan, Yan Wang. (2023) Submicron Nonporous Silica Particles for Enhanced Separation Performance in pCEC. MOLECULES, 28 (8): (3542). [PMID:37110774] [10.3390/molecules28083542] |
| 3. Tingting Wu, Xushun Liu, Hanjun Chen, Ying Liu, Yu Cao. (2022) An in situ exosomal miRNA sensing biochip based on multi-branched localized catalytic hairpin assembly and photonic crystals. BIOSENSORS & BIOELECTRONICS, [PMID:36529054] [10.1016/j.bios.2022.115013] |
| 4. Hui-Zhen Zhang, Mei Shu, Wen-Yu Yang, Hong Pan, Men-Xuan Tang, Yuan-Yang Zhao, Chan Zhong, Guo-Ping Wu. (2022) Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods. LWT-FOOD SCIENCE AND TECHNOLOGY, [10.1016/j.lwt.2022.114251] |
| 5. Can Cheng, Xu Peng, Linjie Xi, Yihao Luo, Yuhang Wang, Yufan Zhou, Xixun Yu. (2022) Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine Achilles tendon and its potential application for anterior cruciate ligament repair. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 111 (2): (170-184). [PMID:36054309] [10.1002/jbm.a.37440] |
| 6. Zhi-Heng Pan, Shi-Song Yu, Chen-Chen Bai, Wen-Yu Yin, Yi-Rong Ma, Zi-Ang Xue, Qin-Yue Lu, Lin-Yi Dong, Xian-Hua Wang. (2022) Poly(caffeic acid)-coated molecularly imprinted magnetic nanoparticles for specific and ultrasensitive detection of glycoprotein. TALANTA, [PMID:35065346] [10.1016/j.talanta.2022.123240] |
| 7. Shuwen Guo, Qiaoxian Huang, Jianwen Wei, Shengpeng Wang, Yitao Wang, Leyong Wang, Ruibing Wang. (2022) Efficient intracellular delivery of native proteins facilitated by preorganized guanidiniums on pillar[5]arene skeleton. Nano Today, [10.1016/j.nantod.2022.101396] |
| 8. Jiasheng Shi, Henan Zhang, Qian Wang, Zhengyu Duan, Li Xu, Fenghao Guo, Yunfeng Xie, Zhiyong Chen. (2021) Biomimetic rigid cryogels with aligned micro-sized tubular structures prepared by conventional redox-induced cryopolymerization in a freezer. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2021.131903] |
| 9. Yu Luo, Shenyu Huang, Lie Ma. (2021) A novel detergent-based decellularization combined with carbodiimide crosslinking for improving anti-calcification of bioprosthetic heart valve. Biomedical Materials, 16 (4): (045022). [PMID:33979785] [10.1088/1748-605X/ac0088] |
| 10. Jie Xing, Fang Wang, Hailin Cong, Song Wang, Youqing Shen, Bing Yu. (2020) Analysis of proteins and chiral drugs based on vancomycin covalent capillary electrophoretic coating. ANALYST, 146 (4): (1320-1325). [PMID:33367313] [10.1039/D0AN02018D] |
| 11. Rui Duan, Chao Peng, Lu Sun, Lu-Xia Zhang, Chen-Chen Bai, Lin-Yi Dong, Xian-Hua Wang. (2020) Integrating boronate affinity controllable-oriented surface imprinting nylon wire and pH-triggered allochroic-graphene oxide for ultrasensitive detection of glycoprotein. SENSORS AND ACTUATORS B-CHEMICAL, [10.1016/j.snb.2020.129310] |
| 12. Yu Luo, Lie Ma. (2020) Bioprosthetic heart valves with reduced immunogenic residuals using vacuum-assisted decellularization treatment. Biomedical Materials, 15 (6): (065012). [PMID:33016260] [10.1088/1748-605X/abaabf] |
| 13. Jie Xing, Ming-Hong Wang, Song Wang, Hai-Lin Cong, You-Qing Shen, Bing Yu. (2020) Analysis of proteins by capillary electrophoresis with a novel diazoresin/β-Cyclodextrin covalent capillary coating method. FERROELECTRICS, [10.1080/00150193.2020.1760608] |
| 14. Qiuyue Sha, Ruixue Guan, Huiying Su, Liang Zhang, Bi-Feng Liu, Zhaoyu Hu, Xin Liu. (2020) Carbohydrate-protein template synthesized high mannose loading gold nanoclusters: A powerful fluorescence probe for sensitive Concanavalin A detection and specific breast cancer cell imaging. TALANTA, [PMID:32797887] [10.1016/j.talanta.2020.121130] |
| 15. Ying Jia, Han Gao, Mengmeng Tong, Donald M. Anderson. (2019) Cell cycle regulation of the mixotrophic dinoflagellate Dinophysis acuminata: Growth, photosynthetic efficiency and toxin production. HARMFUL ALGAE, [PMID:31672228] [10.1016/j.hal.2019.101672] |
| 16. Yameng Han, Zhongju Ye, Fuyan Wang, Tianyu Chen, Lin Wei, Langxing Chen, Lehui Xiao. (2019) Single-particle enumeration-based ultrasensitive enzyme activity quantification with fluorescent polymer nanoparticles. Nanoscale, 11 (31): (14793-14801). [PMID:31353389] [10.1039/C9NR01817D] |
| 17. Wei Chen, Min Fu, Xixi Zhu, Qingyun Liu. (2019) A close-packed imprinted colloidal array for naked-eye detection of glycoproteins under physiological pH. BIOSENSORS & BIOELECTRONICS, [PMID:31306955] [10.1016/j.bios.2019.111499] |
| 18. Yu Luo, Dong Lou, Lie Ma, Changyou Gao. (2019) Optimizing detergent concentration and processing time to balance the decellularization efficiency and properties of bioprosthetic heart valves. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 107 (10): (2235-2243). [PMID:31125175] [10.1002/jbm.a.36732] |
| 19. Manman Han, Wan Li, Rui Chen, Yangyang Han, Xiuhua Liu, Tingting Wang, Huaizhong Guo, Xiaoqiang Qiao. (2018) Amino acid and ionic liquid modified polyhedral oligomeric silsesquioxane-based hybrid monolithic column for high-efficiency capillary liquid chromatography. JOURNAL OF CHROMATOGRAPHY A, [PMID:30153983] [10.1016/j.chroma.2018.08.045] |
| 20. Qi-Qing LI, Jian ZHANG, Ji-Hua LIU, Bo-Yang YU. (2018) Morphological and chemical studies of artificial Andrographis paniculata polyploids. Chinese Journal of Natural Medicines, [PMID:29455732] [10.1016/S1875-5364(18)30033-5] |
| 21. Yi Hao, Ruixia Gao, Dechun Liu, Gaiyan He, Yuhai Tang, Zengjun Guo. (2016) A facile and general approach for preparation of glycoprotein-imprinted magnetic nanoparticles with synergistic selectivity. TALANTA, [PMID:27130111] [10.1016/j.talanta.2016.03.005] |
| 22. Yu Tian, Yunfang Li, Jie Mei, Bo Cai, Jinfeng Dong, Zhiguo Shi, Yuxiu Xiao. (2015) Simultaneous separation of acidic and basic proteins using gemini pyrrolidinium surfactants and hexafluoroisopropanol as dynamic coating additives in capillary electrophoresis. JOURNAL OF CHROMATOGRAPHY A, [PMID:26300480] [10.1016/j.chroma.2015.08.020] |
| 23. Yuanting Xu, Chengcheng Huang, Li Li, Xixun Yu, Xu Wang, Hong Peng, Zhipeng Gu, Yaping Wang. (2013) In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde. CARBOHYDRATE POLYMERS, [PMID:23618251] [10.1016/j.carbpol.2013.03.021] |
| 24. Yuanting Xu, Li Li, Hao Wang, Xixun Yu, Zhipeng Gu, Chengcheng Huang, Hong Peng. (2012) In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation. CARBOHYDRATE POLYMERS, [PMID:23218319] [10.1016/j.carbpol.2012.09.096] |
| 25. Ritian Jin, Chang Liu, Jing Song, Duo Liang, Rong Lin, Zhongyang Ren, Cheng Li, Jiaqi Shang, Shen Yang. (2024) Dynamic gastrointestinal digestion of tilapia (Oreochromis mossambicus) skin gelatin: changes of hydrolysate properties and dipeptidyl peptidase IV inhibitory activity. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, [PMID:39723440] [10.1002/jsfa.14090] |
| 26. Keting Zhou, Yu Xia. (2024) High-Coverage Disulfide Mapping Enabled by Programmable Disulfide-Ene Reaction Integrated onto a Bottom-Up Protein Analysis Workflow. ANALYTICAL CHEMISTRY, [PMID:39425647] [10.1021/acs.analchem.4c04257] |
| 27. Shaofang Ma, Yan Zhang, Zihan Zhu, Deping Wang, Xin Zhou, Jing Wang, Wei Bian, Xinjing Tang. (2024) Nucleolus-Targeting Carbon Dot Nanocomplexes for Combined Photodynamic/Photothermal Therapy. MOLECULAR PHARMACEUTICS, [PMID:39895310] [10.1021/acs.molpharmaceut.4c01211] |
| 28. Xi-Jie Wang, Shuai Lv, Hai-Lin Cong, You-Qing Shen, Qing-Hai Niu, Bing Yu. (2024) Perfluorooctanoic acid used as a new covalently bonded electrophoretic coating to analyze proteins. FERROELECTRICS, [10.1080/00150193.2024.2319555] |
| 29. Qianfu Zhang, Zhixuan Li, Sihan Zhou, Ji Li. (2024) Regenerative Outcomes of Combining siCOL1A2 Hydrogel with Acupuncture in a Rat Model of Chronic Intervertebral Disc Degeneration. Bioengineering-Basel, 11 (11): (1066). [PMID:39593726] [10.3390/bioengineering11111066] |
| 30. Yi Yang, Licheng Yu, Xiaowen Jiang, Yijun Li, Xiwen He, Langxing Chen, Yukui Zhang. (2024) Textural Precursor Compositions Harvested for Independent Signal Generators: Scaling Micron-Sized Flower-Like Metal–Organic Frameworks as Amplifying Units for Dual-Mode Glycoprotein Assay. ANALYTICAL CHEMISTRY, [PMID:38780632] [10.1021/acs.analchem.4c00973] |
| 31. Zihan Zhu, Yan Zhang, Cui He, Yimin Jin, Wei Bian, Xinjing Tang, Jing Wang. (2025) Tumor microenvironment-responsive multinucleated nanocomplexes loaded with carbon dots for combined photothermal/chemodynamic therapy of breast cancer. ChemMedChem, [PMID:39821494] [10.1002/cmdc.202400983] |
| 32. Qin Yang, Zhenkun Zhang, Lin Wang, Xiwen Xing, Jiahai Zhou, Long Li. (2023) Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening. Nanomaterials, 13 (14): (2064). [PMID:37513075] [10.3390/nano13142064] |
| 33. Yujie Zhang, Renling Lu, Ming Chen, Shouyong Zhou, Dabing Zhang, Haifeng Han, Mingliang Zhang, Hongdeng Qiu. (2020) A highly efficient acyl-transfer approach to urea-functionalized silanes and their immobilization onto silica gel as stationary phases for liquid chromatography. JOURNAL OF CHROMATOGRAPHY A, [PMID:32797845] [10.1016/j.chroma.2020.461366] |
| 34. Zihan Zhu, Yan Zhang, Lianggan Luo, Xuan Shang, Xinli Fan, Wei Bian, Jing Wang, Xinjing Tang. (2025) Salivary acid-modified carbon dots loaded with paclitaxel for imaging-guided combination treatment of breast cancer. Biomaterials Science, [PMID:40539237] [10.1039/D5BM00139K] |
| 35. Lu Tian, Baiqiang Chen, Jingxue Huang, Yu Cao, Tingting Wu. (2026) A multifunctional biomimetic interface biosensor for one-step amplified detection of tumor-derived exosomal miRNA. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2026.173463] |
| 36. Tursumamat Nafisa, Liu Qiannan, Lu Jihong, Guo Shuhong, Du Shuangshuang, Liu Shengyang, Wu Shuye, Li Heming, Wu Peng, Li Bin, Wei Juan. (2026) High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes. Nature Communications, 17 (1): (5836). [10.1038/s41467-026-73050-y] |
| 37. Qinghua Cao, Shuai Lv, Yue Wang, Xijie Wang, Hailin Cong, Bing Yu. (2026) Covalent Zwitterionic Peptide-Based Antifouling Coating of the Fused Silica Capillary Applied for CE Separation of Proteins. JOURNAL OF SEPARATION SCIENCE, 49 (7): (e70479). [10.1002/jssc.70479] |
溶液计算器
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