G2213449计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
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有货
为什么选择此级别
用于细胞培养 级 提供 ≥95% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
概述
纤连蛋白（Fibronectin），是细胞外基质中的一种高分子量（~450kDa）糖蛋白，由2个分子质量约220kDa的亚基通过链间二硫键连接而成，广泛分布于机体组织中。在生命科学研究中，具有广泛的应用，比如作为细胞培养基质，提高细胞贴壁率，增强细胞代谢水平，缩短细胞生长时间，另外也可以直接添加至培养基中，促进细胞生长，增殖、维持良好细胞状态。
OsrhFN是一种来源于水稻胚乳细胞的重组人纤连蛋白，相比动物提取来源的纤连蛋白而言，无动物源性，无潜在病毒感染风险，更好的批次稳定性。
规格参数
来源 水稻种子
外观 白色冻干粉
pH值 6.0-8.0
分子量 206kD±5.5kD
内毒素 <0.5EU/μg
CAS No 86088-83-7
注意事项
建议用PBS重新溶解OsrhFN冻干粉，开封后尽快使用。溶解后的OsrhFN冻干粉可至于-20℃保存3-6个月，避免反复冻融。 包装量指的是蛋白的总量，每瓶实际包含“蛋白+保护剂”
应用领域
细胞培养，体外诊断，实验检测，美妆等。
使用方法
A、 铺板/包被：
1、将OsrhFN用PBS 稀释至终参考浓度范围50-100μg/ml，混匀后，加入到孔板或培养瓶中，使OsrhFN液铺满培养瓶/板底。具体OsrhFN添加量参考表1。
2、将加有OsrhFN的培养瓶/板，4℃过夜或者37℃包被1小时；
3、吸净培养瓶/板中的OsrhFN液，收集后请置于4℃保存，1周内可用于其他培养瓶/板包被；
4、取出OsrhFN包被后的培养瓶/板，直接接种细胞即可；
5、如包被后的培养瓶/板暂不使用，请置于4℃密封保存，建议一周内用完。
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表1：不同培养瓶/板包被时推荐OsrhFN加入量
B、细胞培养：
直接加入细胞培养基中，使用终浓度参考范围5-50ug/ml。
使用限制
仅适于科研、实验室和生产使用，不能直接使用于人体
Fibroneptin is a kind of high molecular weight glycoprotein in extracellular matrix. It is composed of two subunits with molecular weight of 220kda connected by disulfide bond, and is widely distributed in the tissue of the body. In the research of life science, it has been widely used, such as as as as as as as as as cell culture medium, improving cell adhesion rate, enhancing cell metabolism level, shortening cell growth time, and directly adding it to the medium to promote cell growth, proliferation and maintain good cell state.
Ostrhfn is a recombinant human fibronectin derived from rice endosperm cells. Compared with the fibronectin extracted from animals, it has no animal origin, no potential risk of virus infection and better batch stability.
Specification parameters
Source Oryza sativa
Appearance white lyophilized powder
PH 6.0-8.0
Molecular weight 206kd ± 5.5kd
Endotoxin < 0.5eu/μ G
CAS No 86088-83-7
Matters needing attention
It is suggested that osrhfn lyophilized powder should be redissolved with PBS and used as soon as possible after unsealing. The dissolved osrhfn lyophilized powder can be stored at - 20 ℃ for 3-6 months to avoid repeated freezing and thawing.
Application
Cell culture, in vitro diagnosis, experimental detection, beauty, etc.
Usage method
A. Planking / coating:
1. Dilute osrhfn with PBS to the final reference concentration range of 50-100μg/ml, mix well, and then add it to the orifice plate or culture bottle to make osrhfn solution cover the bottom of the culture bottle / plate. Refer to table 1 for specific dosage of osrhfn.
2. Culture bottles / plates with osrhfn were coated at 4 ℃ overnight or 37 ℃ for 1 hour;
3. Suck up the osrhfn solution in the culture bottle / plate, and store it at 4 ℃ after collection. It can be used for coating other culture bottles / plates within one week;
4. Take out the culture bottle / plate coated with osrhfn and directly inoculate the cells;
5. If the coated culture bottle / plate is not used for the time being, please keep it at 4 ℃ and use it up within one week.
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Table 1: recommended dosage of osrhfn in different culture bottles / plates
B. Cell culture:
The final concentration reference range was 5-50ug/ml.
Restrictions on use
It is only suitable for scientific research, laboratory and production, not for human body
规格
产品名称
重组人纤维连接蛋白OsrhFN
规格或纯度
用于细胞培养, ≥95%
表达系统
水稻(Oryza sativa)
种属
人(Human)
氨基酸
32-1908 aa
蛋白标签
No tag
CAS编号和信息
86088-83-7
分子类型
蛋白质
储存与运输
物理形态
冻干(Lyophilized)
储存条件
-20°C储存
运输条件
超低温运输
技术文档
高级数据
关联靶点（人）
FN1
Tchem
纤连蛋白(Fibronectin)
(0 活性数据)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
用于细胞培养
目录： 阿拉丁试剂的规格、级别和纯度
Selection Logic, Operating Parameters, and Application Boundaries of Common Cell Culture Coating Solutions
目录： 技术文章
Applicability Comparison of Vitronectin, Laminin and Fibronectin in Cell Culture Coating Systems
目录： 技术文章
Designing Hydrogel Scaffolds as Programmable Cell Microenvironments: From Click-Chemistry Functionalization to 3D Cell Culture Experimental Design
目录： 技术文章
Applicability Comparison of Vitronectin, Laminin and Fibronectin in Cell Culture Coating Systems
目录： 技术文章
Evaluation of Blood-Brain Barrier Permeability: Tracer Extravasation, TEER Measurement, and Interpretation of In Vitro Model Results
目录： 技术文章
Structure and Functions of Fibronectin: Cell Adhesion, Cutaneous Wound Repair, and Topical Applications
目录： 技术文章
How Palmitoyl Pentapeptide-4 Acts on the Skin: From the KTTKS Sequence and Skin Delivery to Collagen Matrix Responses
目录： 技术文章
How Cyclization Changes Peptide Behavior: Conformational Preorganization, Skin Delivery, and Integrin Binding, Using Cyclopeptide-5 as an Example
目录： 技术文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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