ActiBioPure™,His标签,PBS Only,无动物源,无菌,无载体,生物活性,高性能 级 提供 ≥98%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
纤维连接蛋白，也称粘连蛋白，纤连蛋白，英文名称fibronectin（英文缩写FN）是一种高分子质量、多结构域的糖蛋白，在各种细胞-细胞和细胞-基质过程中起着关键作用，如细胞附着、扩散、生长、分化和迁移。它存在于细胞表面，有助于ECM结构。本产品包括细胞结合域，肝磷脂结合域和 CS1 位点三个功能区域。它由 574 个氨基酸组成，分子量 63 kDa。能够大幅地提高逆转录病毒感染哺乳动物细胞的感染效率，其作用原理是：细胞表面 VLA-4 及 VLA-5 可分别与本产品上的 CS-1 位点及细胞结合域结合，逆转录病毒载体可以与肝磷脂结合域结合。在经本产品包被的培养容器表面，细胞与病毒载体以高浓度共存，从而提高基因转染效率。包被在细胞培养皿表面时，显着增强了哺乳动物和昆虫细胞的转染能力。
本产品用重组大肠杆菌基因表达生产人粘连蛋白片段，高纯度，高活性，无动物源性污染。
Fibronectin is a high molecular weight, multi-domain glycoprotein that plays a key role in various cell-cell and cell-matrix processes, such as cell attachment, diffusion, growth, differentiation and migration. It exists on the cell surface and contributes to the ECM structure. This product includes three functional regions: cell binding domain, heparin binding domain and CS1 site. It consists of 574 amino acids and has a molecular weight of 63 kDa. It can greatly improve the infection efficiency of retroviral infection of mammalian cells. Its working principle is: VLA-4 and VLA-5 on the cell surface can bind to the CS-1 site and cell binding domain on this product respectively, and the retroviral vector can bind to the heparin binding domain. On the surface of the culture container coated with this product, cells and viral vectors coexist at high concentrations, thereby improving the efficiency of gene transfection. When coated on the surface of the cell culture dish, the transfection ability of mammalian and insect cells is significantly enhanced.
Recombinant Human Fibronectin Fragment Protein (rp146053) - Protein Bioactivity
Fibronectin Fragment Protein has the effect of promoting cell adhesion. Measured by the ability of the immobilized protein to support the adhesion of NIH/3T3 mouse embryonic fibroblast cells.
Recombinant Human Fibronectin Fragment Protein (rp146053) - SDS-PAGE
2 μg/lane of Recombinant Human Fibronectin Fragment Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 56 kDa.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0521455 |
分析证书 | rp146053 |
| ZJ25F0521454 |
分析证书 | rp146053 |
| ZJ25F0521453 |
分析证书 | rp146053 |
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