级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥90%(SDS-PAGE)
Accession #
Q61526
蛋白标签
C-hFc & His
表达系统
HEK293
内毒素水平
<0.1 EU/μg
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse ErbB3/Her3 Protein (rp166491) at 2 μg/mL can bind Recombinant Human NRG1-beta1Protein, the ED50 for this effect is 60.44 ng/mL.
The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse ErbB3/Her3 Protein (rp166491) at 2 μg/mL can bind Recombinant Human NRG1-beta1Protein, the ED50 for this effect is 60.44 ng/mL.
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。
图片
Recombinant Mouse ErbB3/Her3 Protein (rp166491) - SDS-PAGE 2 μg/lane of Recombinant Mouse ErbB3/Her3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 125 kDa under reducing condition and 270 kDa under non-reducing condition.
Recombinant Mouse ErbB3/Her3 Protein (rp166491) - Protein Bioactivity Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse ErbB3/Her3 Protein (rp166491) at 2 μg/mL can bind Recombinant Human NRG1-beta1Protein, the ED₅₀ for this effect is 60.44 ng/mL.
ZJ25F0723520
危险品化学品经营许可证（带存储）