Measured in a cell proliferation assay using Human HUVEC cells. The ED50 for this effect is <2 ng/mL. The corresponding specific activity is>1×10^6 IU/mg.
N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A.Involved in protecting cells from hypoxia-mediated cell death (By similarity). VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Post-translational: Vascular endothelial growth factor A, long form. Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
生物活性
Measured in a cell proliferation assay using Human HUVEC cells. The ED50 for this effect is <2 ng/mL. The corresponding specific activity is>1×10^6 IU/mg.
Reconstitute in water to a concentration of 1 mg/mL, for other concentrations, please adjust the concentration of phosphate in the solution by yourself.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃~-80℃ (2 years). Upon delivery aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human VEGF 165 Protein (rp166633) - Protein Bioactivity Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1.7 ng/mL.
Recombinant Human VEGF 165 Protein (rp166633) - SDS-PAGE 2 μg/lane of Recombinant Human VEGF 165 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 25 kDa.