Immobilized Recombinant Human CEACAM-5/CD66e Protein (rp181299) at 1.0 μg/mL can bind Cergutuzumab (anti-CEACAM5) (Ab182774) with the EC50 of 9.86 ng/mL.
CEACAM5 又称 CEA 或 CD66e,属于免疫球蛋白超家族中的 CEACAM 大亚家族。CEACAM5 主要由上皮细胞表达,合成为一种分子量为 180 kDa 的糖蛋白,其中 60% 为碳水化合物。CEACAM5 的 N 端含有一个 Ig 样 V 型结构域,其后是六个 Ig 样 C2- 型结构域和一个 GPI 锚点,以同源二聚体形式存在。CEACAM5 和 CEACAM6 在许多癌症中过表达,与粘附和侵袭有关。CEACAM5 可通过同型和异型相互作用介导细胞-细胞粘附。CEACAM5 是一种同型细胞间粘附分子,在肿瘤组织中的表达水平高于相应的正常组织,因此被广泛用作肿瘤标志物。研究还表明,CEACAM5 可抑制细胞分化,从而增加肿瘤的致病性。此外,CEACAM5 还被确定为大肠杆菌 Dr 系列粘附素的宿主受体,大肠杆菌 Dr 粘附素的结合会导致 CEACAM5 同源二聚体的解离。
生物活性
Immobilized Recombinant Human CEACAM-5/CD66e Protein (rp181299) at 1.0 μg/mL can bind Cergutuzumab (anti-CEACAM5) (Ab182774) with the EC50 of 9.86 ng/mL.
89.4-193.9 kDa, under reducing conditions; 81.4-179.6 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 8% Trehalose, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human CEACAM-5/CD66e Protein (rp181299) - ELISA Immobilized Recombinant Human CEACAM-5/CD66e Protein (rp181299) at 1.0 μg/mL can bind Cergutuzumab (anti-CEACAM5) (Ab182774) with the EC50 of 9.86 ng/mL.
Recombinant Human CEACAM-5/CD66e Protein (rp181299) - SDS-PAGE 5 μg/lane of Recombinant Human CEACAM-5/CD66e Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 89.4-193.9 kDa under reducing conditions and 81.4-179.6 kDa under non-reducing conditions.