Recombinant Human Cathepsin B Protein

Name: Recombinant Human Cathepsin B Protein
SKU: rp183609
Availability: InStock

货号: rp183609

COA

有货

级别和纯度: 无动物源 ? 无载体 ? 生物活性 ? ActiBioPure™ ? His标签 ? PBS Only ? ≥95%(SDS-PAGE)

Accession #

P07858

蛋白标签

C-His

表达系统

HEK293

内毒素水平

<1.0 EU/μg

生物活性

Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.

规格

库存

价格

数量

10μg

rp183609-10μg

现货

¥1,099.90

50μg

rp183609-50μg

现货

¥3,199.90

100μg

rp183609-100μg

现货

¥4,799.90

1mg

rp183609-1mg

期货

¥16,999.90

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概述规格储存与运输图片高级数据质检证书(CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器常见问题

概述

Protein Function:
Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

规格

产品名称

Recombinant Human Cathepsin B Protein

别名

重组人Cathepsin B蛋白 | 重组人组织蛋白酶B蛋白

英文别名

规格或纯度

无动物源,无载体,生物活性,ActiBioPure™,His标签,PBS Only,≥95%(SDS-PAGE)

生物活性

Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.

表达系统

HEK293

种属

人(Human)

氨基酸

1-339 aa

序列

MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKIHHHHHHHHHH

蛋白标签

C-His

无载体

Yes

无动物源

Yes

来源

重组表达

预测分子量

37.3 kDa

SDS-PAGE

40.2-45.0 kDa, under reducing conditions; 37.0-44.0 kDa, under non-reducing conditions

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, pH 7.4

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

在 -20°C 下可保存 1 年。避免冷冻/解冻循环。

图片

ELISA: Recombinant Human Cathepsin B Protein (rp183609)

Recombinant Human Cathepsin B Protein (rp183609) - ELISA
Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.

SDS-PAGE: Recombinant Human Cathepsin B Protein (rp183609)

Recombinant Human Cathepsin B Protein (rp183609) - SDS-PAGE
3 μg/lane of Recombinant Human Cathepsin B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40.2-45.0 kDa under reducing conditions and 37.0-44.0 kDa under non-reducing conditions.

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0708016	分析证书	rp183609
ZJ24F0708017	分析证书	rp183609
ZJ24F0708018	分析证书	rp183609

技术文档和文章

阿拉丁®靶点蛋白

目录：技术文章

探索主题:

药物开发信号通路细胞因子靶点蛋白药物靶点受体免疫检查点细胞表面标记物

组织蛋白酶家族分类与疾病机制：Cathepsin B、D、K、L、S、G 的活性调控和研究应用

目录：技术文章

探索主题:

骨吸收抗原呈递组织蛋白酶炎症小体 Cathepsin Cathepsin B Cathepsin D Cathepsin K Cathepsin L Cathepsin S Cathepsin G CTSB CTSD CTSK CTSL CTSS CTSG 溶酶体蛋白酶组织蛋白酶抑制剂蛋白酶活性检测肿瘤侵袭

Classification and Disease Mechanisms of the Cathepsin Family: Activity Regulation and Research Applications of Cathepsin B, D, K, L, S, and G

目录：技术文章

探索主题:

antigen presentation Bone resorption inflammasome Cathepsin Cathepsin B Cathepsin D Cathepsin K Cathepsin L Cathepsin S Cathepsin G CTSB CTSD CTSK CTSL CTSS CTSG Lysosomal protease Cathepsin inhibitor Protease activity assay Tumor invasion

Aladdin® Target Proteins

目录：技术文章

探索主题:

Cytokines Drug development Target Proteins Drug Targets Receptors Immune Checkpoints Cell Surface Markers Signaling Pathways

半胱氨酸内肽酶的功能分型与实验应用：植物蛋白酶、组织蛋白酶和Arg位点内切酶

目录：技术文章

探索主题:

木瓜蛋白酶 clostripain 组织蛋白酶 Cathepsin B Cathepsin K 半胱氨酸内肽酶无花果蛋白酶 Arg-C

Functional Classification and Experimental Applications of Cysteine Endopeptidases: Plant Proteases, Cathepsins, and Arg-Specific Endoproteases

目录：技术文章

探索主题:

papain clostripain Cathepsin Cathepsin B Cathepsin K Arg-C Cysteine Endopeptidase Ficin

胰腺炎动物模型构建与评价：急性炎症、慢性纤维化和病因模拟研究

目录：技术文章

探索主题:

动物造模 L-精氨酸胰腺炎模型急性胰腺炎慢性胰腺炎雨蛙素牛磺胆酸钠胰酶活化胰腺纤维化

Construction and Evaluation of Pancreatitis Animal Models: Acute Inflammation, Chronic Fibrosis, and Etiological Simulation Research

目录：技术文章

探索主题:

Animal Modeling L-arginine Pancreatitis Model Acute Pancreatitis Chronic Pancreatitis Cerulein Sodium Taurocholate Pancreatic Enzyme Activation Pancreatic Fibrosis

溶酶体在代谢和自身免疫性疾病中的作用

目录：技术文章

探索主题:

炎症自噬溶酶体内溶酶体系统 TFEB TFE3 mTORC1 LAMP1 LAMP2A CMA 自身免疫代谢疾病

The Role of Lysosomes in Metabolic and Autoimmune Diseases

目录：技术文章

探索主题:

Inflammation metabolic diseases Autophagy Lysosome TFEB TFE3 mTORC1 LAMP1 LAMP2A CMA Endolysosomal System Autoimmunity

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

常见问题

本产品的纯度是多少？如何测定？

本产品的纯度为 ≥95%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。

什么是「无动物源」？

「无动物源」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

本产品应如何储存？

请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。

什么是「无载体」？

「无载体」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

本产品如何运输？

本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。

如何获取批次专用COA？

每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Bioactive 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 His Tag 级别指南 → 查看 PBS Only 级别指南 →

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Recombinant Human Cathepsin B Protein

概述

规格

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

常见问题

需要帮助选择级别？

查看相关“主题”系列