Recombinant Human Cathepsin B Protein

货号: rp183609
有货
级别和纯度: 无动物源 ? 无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥95%(SDS-PAGE)
Accession #
P07858
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.
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规格
库存
价格
数量
10μg
rp183609-10μg
现货 Stock Image
¥1,099.90
50μg
rp183609-50μg
现货 Stock Image
¥3,199.90
100μg
rp183609-100μg
现货 Stock Image
¥4,799.90
1mg
rp183609-1mg
期货 Stock Image
¥16,999.90
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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无动物源,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Protein Function:
Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

规格

产品名称
Recombinant Human Cathepsin B Protein
别名
重组人Cathepsin B蛋白 | 重组人组织蛋白酶B蛋白
英文别名
APPS | Cathepsin B | Cathepsin B1 | CPSBamyloid precursor protein secretase | CTSB | cysteine protease | EC 3.4.22 | EC 3.4.22.1 | APP secretase
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,His标签,PBS Only,≥95%(SDS-PAGE)
生物活性
Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.
表达系统
HEK293
种属
人(Human)
氨基酸
1-339 aa
序列
MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKIHHHHHHHHHH
蛋白标签
C-His
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
37.3 kDa
SDS-PAGE
40.2-45.0 kDa, under reducing conditions; 37.0-44.0 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片

Recombinant Human Cathepsin B Protein (rp183609) - ELISA
Immobilized Recombinant Human Cathepsin B Protein (rp183609) at 1.0 μg/mL can bind Cathepsin B Mouse mAb (Ab093155) with the EC50 of 163.1 ng/mL.

Recombinant Human Cathepsin B Protein (rp183609) - SDS-PAGE
3 μg/lane of Recombinant Human Cathepsin B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40.2-45.0 kDa under reducing conditions and 37.0-44.0 kDa under non-reducing conditions.

技术文档

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高级数据

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0708016 分析证书 rp183609
ZJ24F0708017 分析证书 rp183609
ZJ24F0708018 分析证书 rp183609
技术文档和文章
阿拉丁®靶点蛋白
组织蛋白酶家族分类与疾病机制:Cathepsin B、D、K、L、S、G 的活性调控和研究应用
Classification and Disease Mechanisms of the Cathepsin Family: Activity Regulation and Research Applications of Cathepsin B, D, K, L, S, and G
Aladdin® Target Proteins
半胱氨酸内肽酶的功能分型与实验应用:植物蛋白酶、组织蛋白酶和Arg位点内切酶
Functional Classification and Experimental Applications of Cysteine Endopeptidases: Plant Proteases, Cathepsins, and Arg-Specific Endoproteases
胰腺炎动物模型构建与评价:急性炎症、慢性纤维化和病因模拟研究
Construction and Evaluation of Pancreatitis Animal Models: Acute Inflammation, Chronic Fibrosis, and Etiological Simulation Research
溶酶体在代谢和自身免疫性疾病中的作用
The Role of Lysosomes in Metabolic and Autoimmune Diseases
溶液计算器

常见问题

本产品的纯度是多少?如何测定?
本产品的纯度为 ≥95%,采用SDS-PAGE测定。批次的具体数值以分析证书(COA)为准。
什么是「无动物源」?
「无动物源」表示该物料经过加工和检测,适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如,电子应用的金属含量、电池应用的粒度、光学应用的折射率,或对水分敏感工艺的水分含量。
本产品应如何储存?
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
什么是「无载体」?
「无载体」表示该物料经过加工和检测,适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如,电子应用的金属含量、电池应用的粒度、光学应用的折射率,或对水分敏感工艺的水分含量。
本产品如何运输?
本产品采用保温箱配冰袋运输。收货后请立即拆包,并按上述储存条件保存。
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每个订单都会随附分析证书(COA),您也可以从账户订单历史中下载。如需订购前的COA,请联系您的客户代表,或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。