计算溶液所需的质量、体积或浓度。
可溶性果胶 (SP)提取试剂
有货
为什么选择此级别
BioReagent,植物样品抽提用 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
天然果胶类物质以原果胶、果胶 (Pectin)、果胶酸的形态广泛存在于植物的果实、根、茎、叶中，是细胞壁的一种组成成分，它们伴随纤维素而存在，构成相邻细胞中间层粘结物，使植物组织细胞紧紧黏结在一起。原果胶是不溶于水的物质，但可在酸、碱、盐等化学试剂及酶的作用下，加水分解转变成水溶性果胶。果胶 (Pectin)又称多聚半乳糖醛酸，是由D-半乳糖醛酸以α-1,4糖苷键连接形成的直链状聚合物，本质上是一种线形的多糖聚合物，含有数百至约1000个脱水半乳糖醛酸残基，其相应的平均相对分子质量为50000-150000。
可溶性果胶 (SP)提取试剂主要用于提取植物组织或果实中可溶性果胶。该试剂仅用于科研领域，不宜用于临床诊断或其他用途。
自备材料：
1、蒸馏水
2、实验材料：桃子、李子、苹果、杏等果实或其他植物组织
3、天平、剪刀、研钵或匀浆器、离心机、水浴锅、试管或离心管
操作步骤 (仅供参考)：
1、取果实或其他植物组织，洗净擦干，称取剪碎的新鲜样品0.2g，置于研钵或匀浆器。
2、加入1-2ml可溶性果胶提取试剂，充分研磨或匀浆后转入10ml试管中。用可溶性果胶提取试剂冲洗研钵或匀浆器并转移至试管中，补加可溶性果胶提取试剂至10ml。
3、沸水浴30min，在煮沸过程中及时补加可溶性果胶提取试剂至10ml，取出冷却至室温，8000r/min离心15min，弃上清。重复该步骤2次，以去除样品中的糖分及其他物质。
4、取含有沉淀的试管，加入4ml蒸馏水，50℃水浴30min以溶解果胶。取出冷却至室温，8000r/min离心15min，将上清液转移至新试管中。用少量蒸馏水洗涤沉淀，8000r/min再次离心15min，一并将上清液转移至上述新试管中，加蒸馏水定容至10ml，即为可溶性果胶提取液。
注意事项：
1、取样量、试剂用量应根据果胶含量适当调整。
2、可溶性糖对测定结果有较大影响，应彻底去除样品中的可溶性糖。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Natural pectin substances exist extensively in the fruits, roots, stems and leaves of plants in the forms of protopectin, pectin and pectic acid. As a component of plant cell walls, they coexist with cellulose and form the intercellular cement between adjacent cells, thus tightly binding plant tissue cells together. Protopectin is a water-insoluble substance, but it can be hydrolyzed into water-soluble pectin under the action of chemical reagents such as acids, alkalis, salts and enzymes. Pectin, also known as polygalacturonic acid, is a linear polymer formed by the linkage of D-galacturonic acid via α-1,4 glycosidic bonds. Essentially, it is a linear polysaccharide polymer containing hundreds to about 1,000 anhydrogalacturonic acid residues, with a corresponding average relative molecular mass ranging from 50,000 to 150,000.
This Soluble Pectin (SP) Extraction Reagent is mainly used for extracting soluble pectin from plant tissues or fruits. Note: This reagent is intended for research purposes only and not for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Distilled water
2. Experimental materials: Fruits such as peaches, plums, apples, apricots, or other plant tissues
3. Balance, scissors, mortar or homogenizer, centrifuge, water bath, test tubes or centrifuge tubes
Operating Procedures (For Reference Only)
1. Take the fruits or other plant tissues, rinse and dry them thoroughly, weigh 0.2 g of the cut fresh samples, and place them in a mortar or homogenizer.
2. Add 1-2 ml of the Soluble Pectin Extraction Reagent, grind or homogenize the mixture thoroughly, and then transfer it into a 10 ml test tube. Rinse the mortar or homogenizer with the Soluble Pectin Extraction Reagent and transfer the rinsing solution into the test tube, then add additional Soluble Pectin Extraction Reagent to make the total volume up to 10 ml.
3. Place the test tube in a boiling water bath for 30 min. During boiling, timely replenish the Soluble Pectin Extraction Reagent to maintain the volume at 10 ml. Take out the test tube and cool it down to room temperature. Centrifuge the mixture at 8,000 r/min for 15 min and discard the supernatant. Repeat this step twice to remove sugars and other substances from the sample.
4. Take the test tube containing the precipitate, add 4 ml of distilled water, and place it in a 50℃ water bath for 30 min to dissolve the pectin. Take out the test tube and cool it to room temperature. Centrifuge the mixture at 8,000 r/min for 15 min and transfer the supernatant into a new test tube. Wash the precipitate with a small amount of distilled water, centrifuge again at 8,000 r/min for 15 min, transfer the supernatant into the aforementioned new test tube, and add distilled water to make the total volume up to 10 ml. This is the soluble pectin extract.
Precautions
1. The sampling amount and reagent dosage should be adjusted appropriately according to the pectin content.
2. Soluble sugars have a significant impact on the determination results, so it is necessary to completely remove soluble sugars from the sample.
3. Please use the reagent as soon as possible after opening to avoid affecting the subsequent experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
可溶性果胶提取试剂 | SP提取试剂
英文别名
Soluble Pectin Extraction Reagent | SP Extraction Reagent
规格或纯度
BioReagent, 植物样品抽提用
稳定性与储存
Store at room temperature long term (24 months).
英文名称
Soluble Pectin (SP) Extraction Reagent
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332853 |
分析证书 | S1509597 |
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