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BioReagent,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温,干燥。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
半固体营养琼脂培养基(Semisolid Nutrient Agar)是一款通用型基础微生物培养基,以营养琼脂为基础,通过降低琼脂添加量制成半固体凝胶状态,兼顾流动性与支撑性,是微生物实验室常规检测、菌种管理及生化试验的核心耗材。本品为淡黄色粉末状干粉培养基,配比科学、溶解性好、成品澄清度高,可稳定满足各类细菌的培养、动力检测与短期保藏需求,广泛适配食品、医药、水质、畜牧、科研院校等多领域微生物检测场景,符合国标及行业通用检测标准。也用于动力观察,菌种保存,H抗原位相变异试验。
原理和用途:
蛋白胨和牛肉浸出粉提供氮源、生长所必需的维生素、矿物质和氨基酸等;氯化钠维持均衡的渗透压;琼脂是凝固剂。
配方:(g/L)
| 蛋白胨 | 10.0 g |
| 牛肉浸出粉 | 3.0 g |
| 琼脂 | 4.0 g |
| 氯化钠 | 5.0 g |
| 最终pH值 (25°C) | 7.2±0.2 |
配制方法:
称取本品22.0g,置于1L纯化水中。加热煮沸至完全溶解,分装试管,121°C高压灭菌15min,直立凝固,备用。
质量控制:
| 测试项目 | 标准指标 |
| 外观 | 粉末状 |
| 干粉颜色 | 淡黄色 |
| 制备好培养基颜色 | 淡黄色半固体 |
| 最终pH值 (25°C) | 7.2±0.2 |
微生物测试:
根据《中华人民共和国药典》检测:大肠埃希氏菌、蜡样芽孢杆菌和福氏志贺氏菌。
| 质控菌株及编号 | 生长情况/菌落特征 |
| 大肠埃希氏菌CMCC(B)44102 | 生长良好,扩散生长 |
| 蜡样芽孢杆菌CMCC(B)63303 | 生长良好,扩散生长 |
| 福氏志贺氏菌CMCC(B)51572 | 生长良好,沿穿刺线生长 |
接种条件:定性。
培养条件:35°C,24小时。
注意事项:
1. 称取培养基时,请做好必要的防护措施,以免引起身体不适。
2. 培养基应置于避光、干燥处保存,使用后应立即旋紧瓶盖,以免吸潮结块。
3. 使用后的培养基应在121°C下高压灭菌30分钟后处理。
4. 本品不得用于临床检测。
Semisolid Nutrient Agar is a universal basic microbiological medium. Prepared based on nutrient agar with a reduced agar dosage to form a semisolid gel, it balances fluidity and support performance. It serves as a core consumable for routine microbial testing, strain preservation and biochemical assays in microbiology laboratories. This product is a pale yellow powdered dehydrated culture medium with a well-balanced formula, excellent solubility and high clarity of prepared medium. It stably supports the cultivation, motility testing and short-term preservation of various bacteria. It is widely applied to microbial detection in food, pharmaceutical, water quality, animal husbandry, scientific research institutes and other fields, complying with national standards and general industrial testing specifications. It is also used for motility observation, strain storage and H antigen phase variation tests.
Principle and Purpose:
Peptone and beef extract powder supply nitrogen sources, essential vitamins, minerals, amino acids and other nutrients required for microbial growth; sodium chloride maintains balanced osmotic pressure; agar acts as the gelling agent.
Formula: (g/L)
| Peptone | 10.0 g |
| Beef Extract Powder | 3.0 g |
| Agar | 4.0 g |
| Sodium Chloride | 5.0 g |
| Final pH (25°C) | 7.2±0.2 |
Preparation Method:
Weigh 22.0 g of this product and add into 1 L of purified water. Heat and boil until completely dissolved, dispense into test tubes, autoclave at 121°C for 15 minutes, place upright to solidify, then set aside for use.
Quality Control:
| Test Item | Standard Index |
| Appearance | Powder |
| Color of dehydrated medium | Pale yellow |
| Color of prepared medium | Pale yellow semisolid |
| Final pH (25°C) | 7.2±0.2 |
Microbiological Testing:
Testing items are detected in accordance with the Pharmacopoeia of the People's Republic of China: Escherichia coli, Bacillus cereus and Shigella flexneri.
| Quality Control Strains and Strain Numbers | Growth Status / Colony Characteristics |
| Escherichia coli CMCC(B)44102 | Good growth with diffuse spreading growth |
| Bacillus cereus CMCC(B)63303 | Good growth with diffuse spreading growth |
| Shigella flexneri CMCC(B)51572 | Good growth, growing only along the stab line |
Inoculation mode: Qualitative test.
Incubation conditions: 35 °C, 24 hours
Precautions:
1. Wear necessary protective equipment when weighing the medium to prevent physical discomfort.
2. Store the medium in a dry and dark place. Tighten the cap immediately after use to avoid moisture absorption and caking.
3. Discard used medium only after autoclaving at 121 °C for 30 minutes.
4. This product shall not be used for clinical testing.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1523288 |
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