计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥60% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温,干燥。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 53 篇同行评审文献引用。
概述
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Althoμgh Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.A viability dye for quantifying live versus dead cells
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
A viability dye for quantifying live versus dead cells
规格
别名
台盼蓝
英文别名
Centraline Blue 3B | Bleue diretto 3B | Modr Prima 14 [Czech] | Sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate | Benzanil Blue R | Congoblau 3B | Dianilblau H3G | TRYPAN BLUE [MART.] | Diaminblau 3B | NCGC00167554-01 | TRYPAN BLUE [VANDF] | Bl
规格或纯度
≥60%
英文名称
Trypan Blue
储存条件
室温,干燥
运输条件
常规运输
纯度
≥60%
名称和识别符
PubChem SID
EC号
200-786-7
分子类型
小分子
IUPAC Name
tetrasodium;5-amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate
INCHI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChi Key
GLNADSQYFUSGOU-UHFFFAOYSA-J
Smiles
CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
Isomeric SMILES
CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
分子量
960.81
Beilstein号
4360496
技术文档
高级数据
系统学分类
分类树（Taxonomy Tree）
| 界（kingdom) | 有机化合物 |
|---|---|
| 超类（Superclass) | 苯类化合物 |
| 类（Class) | 苯及其取代衍生物 |
| 亚类（Subclass） | 联苯及其衍生物 |
| 中间层级节点（Intermediate Tree Nodes） | 联苯胺 |
| 直接上位类(Direct Parent) | 3,3'-二取代联苯胺 |
| 其他上位类（Alternative Parents） | 2-萘磺酸及其衍生物 2-萘磺酸盐 1-磺基,2-未取代芳香族化合物 甲苯 酚类化合物 磺酰基 有机磺酸 偶氮化合物 炔丙基型1,3-偶极有机化合物 伯胺 有机氧化合物 有机钠盐 有机氧化物 碳氢化合物衍生物 有机阳离子 |
| 分子骨架（Molecular Framework) | 芳香族同多环化合物 |
| 取代基（Substituents） | 1-磺酰基-2-未取代芳香化合物 - 2-萘磺酸 - 2-萘磺酸或其衍生物 - 3,3'-二取代联苯胺 - 氨基酸 - 芳香族同多环化合物 - 芳基磺酸或其衍生物 - 偶氮化合物 - 烃衍生物 - 萘 - 萘磺酸盐 - 萘磺酸或其衍生物 - 有机1,3-双极性化合物 - 有机碱金属盐 - 有机铵盐 - 有机硝基化合物 - 有机氧化物 - 有机氧化合物 - 有机盐 - 有机钠盐 - 有机磺酸或其衍生物 - 有机氮化合物 - 有机氧化合物 - 有机硒化合物 - 有机磺酸或其衍生物 - 有机硫化合物 - 苯氧基化合物 - 初级胺 - 丙炔型1,3-双极性有机化合物 - 磺酰基 - 甲苯 |
| 描述（Description） | 该化合物属于有机化合物中的3,3'-二取代苯二胺类。这类化合物具有苯二胺骨架，仅在3-位和3'-位发生取代。 |
| 外部描述符（External Descriptors） | 暂无 |
三维结构
交互式化学结构模型
化学和物理性质
溶解性
Soluble in water (10 mg/ml at 25 °C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
熔点
>300°C
分子量
960.800 g/mol
XLogP3
氢键供体数Hydrogen Bond Donor Count
4
氢键受体数Hydrogen Bond Acceptor Count
20
可旋转键计数Rotatable Bond Count
5
精确质量Exact Mass
959.982 Da
单同位素质量Monoisotopic Mass
959.982 Da
拓扑极表面积Topological Polar Surface Area
404.000 Ų
重原子数Heavy Atom Count
62
形式电荷Formal Charge
0
复杂度Complexity
1790.000
同位素原子数Isotope Atom Count
0
定义的原子立体中心计数Defined Atom Stereocenter Count
0
未定义的原子立体中心计数Undefined Atom Stereocenter Count
0
定义的键立体中心计数Defined Bond Stereocenter Count
0
未定义的键立体中心计数Undefined Bond Stereocenter Count
0
所有立体化学键的总数The total count of all stereochemical bonds
0
共价键合单元计数Covalently-Bonded Unit Count
5
安全和危险性（GHS）
象形图
信号词
危险
危险声明
H350: 可能导致癌症
预防措施声明
P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。
P405: 密闭存放
P501: 将内容物/容器处理到。。。
P203: 使用前，获取、阅读并遵守所有安全说明。
P318: 如果暴露或担心，请就医。
WGK Germany
3
RTECS
QJ6475000
Merck Index
9792
个人防护装备
Eyeshields,full-face particle respirator type N100 (US),Gloves,respirator cartridge type N100 (US),type P1 (EN143) respirator filter,type P3 (EN 143) respirator cartridges
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 K1808014 |
分析证书 | T108743 |
| E2625614 |
分析证书 | T108743 |
| E2224059 |
分析证书 | T108743 |
| E2224099 |
分析证书 | T108743 |
| A2621642 |
分析证书 | T108743 |
| G2003051 |
分析证书 | T108743 |
| H2323035 |
分析证书 | T108743 |
| B2302724 |
分析证书 | T108743 |
| B2302726 |
分析证书 | T108743 |
| B2302727 |
分析证书 | T108743 |
| B2302725 |
分析证书 | T108743 |
| B2302728 |
分析证书 | T108743 |
| B2302729 |
分析证书 | T108743 |
| E2224080 |
分析证书 | T108743 |
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此产品的引用文献
引用文献
| 1. Kankan Chen, Jiawei Xu, Jingjing Song, Zhonggang Liu, Zheng Guo. (2023) A facile ratiometric electrochemical sensing platform with in-situ electropolymerized thionine on effective sensing of 5-hydroxytryptamine. ELECTROANALYSIS, 36 (3): (e202300324). [10.1002/elan.202300324] |
| 2. Guangyong Zeng, Hu Zheng, Kun Zhou, Hao Shi, Size Zheng, Hui Ma, Peng Wang, Shengyan Pu. (2023) Preparation, Characterization and Application of Novel Photocatalytic Two-Dimensional Material Membrane: A Reform of Comprehensive Experimental Teaching. Sustainability, 15 (14): (11342). [10.3390/su151411342] |
| 3. He Zhang, Wenmei Luo, Yuping Du, Hao Shi, Guangyong Zeng, Xinxing Yan, Xiaoke Li. (2023) The g-C3N4 decorated carbon aerogel with integrated solar steam generation and photocatalysis for effective desalination and water purification. DESALINATION, [10.1016/j.desal.2023.116821] |
| 4. Ling Jiang, Huan Huang, Xingyu Shi, Jian Wu, Juexian Ye, Qian Xu, Shaobin Fang, Chuanbin Wu, Rui Luo, Chao Lu, Daojun Liu. (2023) Biocontrol Microneedle Patch: A Promising Agent for Protecting Citrus Fruits from Postharvest Infection. Pharmaceutics, 15 (4): (1219). [PMID:37111704] [10.3390/pharmaceutics15041219] |
| 5. Xin-Lei Cheng, Ting-Rui Fu, Dan-Feng Zhang, Jian-Hua Xiong, Wu-Ying Yang, Juan Du. (2023) Biomass-assisted fabrication of rGO-AuNPs as surface-enhanced Raman scattering substrates for in-situ monitoring methylene blue degradation. ANALYTICAL BIOCHEMISTRY, [PMID:36858251] [10.1016/j.ab.2023.115087] |
| 6. Yujiao Tu, Ze Liu, Lei Jiang, Yingying Xiang, Fei Song, Lifen Meng, Xiuling Ji, Lin Yuan. (2022) Green ratiometric fluorescent dual-mode nanosensor for highly selective and sensitive detection of new coccine in food. DYES AND PIGMENTS, [10.1016/j.dyepig.2022.111024] |
| 7. Wang Yongtao, Wang Nana, Yang Yingjun, Chen Yazhou, Zhang Zhengguo. (2022) Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma. JOURNAL OF NANOBIOTECHNOLOGY, 20 (1): (1-13). [PMID:36424661] [10.1186/s12951-022-01713-1] |
| 8. Lin Yuan, Lei Jiang, Ze Liu, Yingying Xiang, Fei Song, Yujiao Tu. (2022) Selective and sensitive simultaneous detection of tartrazine and Hg2+ based on N-doped yellow-green fluorescent carbon dots. DYES AND PIGMENTS, [10.1016/j.dyepig.2022.110893] |
| 9. Jin Wen, Xiaoke Li, He Zhang, Size Zheng, Caini Yi, Liu Yang, Jinwen Shi. (2022) Architecting Janus hydrogel evaporator with polydopamine-TiO2 photocatalyst for high-efficient solar desalination and purification. SEPARATION AND PURIFICATION TECHNOLOGY, [10.1016/j.seppur.2022.122403] |
| 10. Yingying Xiang, Yujiao Tu, Lei Jiang, Lin Yuan, Ze Liu, Qiang Xie, Xuequan Xiong, Fei Song. (2022) Orange fluorescent dual-mode nanoprobe for selective and sensitive detection of amaranth based on S,N-Doped carbon dots. DYES AND PIGMENTS, [10.1016/j.dyepig.2022.110533] |
| 11. Siyuan Huang, Qi Su, Xiaoqiang Hou, Kuankuan Han, Shufang Ma, Bingshe Xu, Yingjun Yang. (2022) Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles. Frontiers in Bioengineering and Biotechnology, [PMID:35711638] [10.3389/fbioe.2022.922159] |
| 12. Xiaojie Cheng, Jiahui Liao, Yue Xue, Qingquan Lin, Zhaomei Yang, Guilong Yan, Guangyong Zeng, Arijit Sengupta. (2021) Ultrahigh-flux and self-cleaning composite membrane based on BiOCl-PPy modified MXene nanosheets for contaminants removal from wastewater. JOURNAL OF MEMBRANE SCIENCE, [10.1016/j.memsci.2021.120188] |
| 13. Song Zhu, Fei Luo, Bin Zhu, Fei Ling, Er-Long Wang, Tian-Qiang Liu, Gao-Xue Wang. (2021) A Nanobody-Mediated Virus-Targeting Drug Delivery Platform for the Central Nervous System Viral Disease Therapy. Microbiology Spectrum, 9 (3): [PMID:34817277] [10.1128/Spectrum.01487-21] |
| 14. Shanshan Wang, Hufei Wang, Yanqiu Chen, Jilai Liu, Xiaozhen He, Da Huang, Yuanzi Wu, Yonghai Chen, Zuquan Weng. (2021) Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity. TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, [10.1080/02772248.2021.1951731] |
| 15. Sihao Pan, Jiaoyu Ren, Enhui Ma, Ke Wang, Sicheng Yang, Hong Wang. (2021) Dual-Propelled Sporopollenin-Exine-Capsule Micromotors for Near-Infrared Light Triggered Degradation of Organic Pollutants. ChemNanoMat, 7 (4): (483-487). [10.1002/cnma.202100011] |
| 16. Jing Zhou, Dan Yang, Guohui Liu, Siying Li, Wennan Feng, Guocheng Yang, Jin He, Yuping Shan. (2021) Highly sensitive detection of DNA damage in living cells by SERS and electrochemical measurements using a flexible gold nanoelectrode. ANALYST, 146 (7): (2321-2329). [PMID:33623934] [10.1039/D1AN00220A] |
| 17. Beibei Yang, Yating Dong, Yifeng Shen, Ailin Hou, Guilan Quan, Xin Pan, Chuanbin Wu. (2021) Bilayer dissolving microneedle array containing 5-fluorouracil and triamcinolone with biphasic release profile for hypertrophic scar therapy. Bioactive Materials, [PMID:33553824] [10.1016/j.bioactmat.2021.01.014] |
| 18. Chi Zhang, Fan Gao, Wei Wu, Wen-Xiu Qiu, Lu Zhang, Runqing Li, Ze-Nan Zhuang, Wuyang Yu, Han Cheng, Xian-Zheng Zhang. (2019) Enzyme-Driven Membrane-Targeted Chimeric Peptide for Enhanced Tumor Photodynamic Immunotherapy. ACS Nano, [PMID:31566945] [10.1021/acsnano.9b04315] |
| 19. Hong Cheng, Ping Yuan, Guiling Fan, Linping Zhao, Rongrong Zheng, Bin Yang, Xiaozhong Qiu, Xiyong Yu, Shiying Li, Xianzheng Zhang. (2019) Chimeric peptide nanorods for plasma membrane and nuclear targeted photosensitizer delivery and enhanced photodynamic therapy. Applied Materials Today, [10.1016/j.apmt.2019.04.017] |
| 20. Maria Wasim, Aneela Sabir, Muhammad Shafiq, Rafi Ullah Khan. (2019) Fractionation of direct dyes using modified vapor grown carbon nanofibers and zirconia in cellulose acetate blend membranes. SCIENCE OF THE TOTAL ENVIRONMENT, [PMID:31055100] [10.1016/j.scitotenv.2019.04.351] |
| 21. Xi Chen, Yi He, Yi Fan, Guangyong Zeng, Liyun Zhang. (2018) Nature-inspired polyphenol chemistry to fabricate halloysite nanotubes decorated PVDF membrane for the removal of wastewater. SEPARATION AND PURIFICATION TECHNOLOGY, [10.1016/j.seppur.2018.11.036] |
| 22. Zu-Sheng Huang, Yun-Yun Quan, Jia-Jun Mao, Yi-Li Wang, Yuekun Lai, Jing Zheng, Zhong Chen, Kun Wei, Huaqiong Li. (2018) Multifunctional superhydrophobic composite materials with remarkable mechanochemical robustness, stain repellency, oil-water separation and sound-absorption properties. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2018.10.123] |
| 23. Yingqing Zhan, Xinyi Wan, Shuangjiang He, Qiangbin Yang, Yi He. (2017) Design of durable and efficient poly(arylene ether nitrile)/bioinspired polydopamine coated graphene oxide nanofibrous composite membrane for anionic dyes separation. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2017.09.147] |
| 24. Guangyong Zeng, Zhongbin Ye, Yi He, Xi Yang, Jing Ma, Heng Shi, Ziliang Feng. (2017) Application of dopamine-modified halloysite nanotubes/PVDF blend membranes for direct dyes removal from wastewater. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2017.04.131] |
| 25. Shi-Ying Li, Wen-Xiu Qiu, Hong Cheng, Fan Gao, Feng-Yi Cao, Xian-Zheng Zhang. (2017) A Versatile Plasma Membrane Engineered Cell Vehicle for Contact-Cell-Enhanced Photodynamic Therapy. ADVANCED FUNCTIONAL MATERIALS, 27 (12): (1604916). [10.1002/adfm.201604916] |
| 26. Shi-Ying Li, Hong Cheng, Wen-Xiu Qiu, Li-Han Liu, Si Chen, Ying Hu, Bo-Ru Xie, Bin Li, Xian-Zheng Zhang. (2015) Protease-Activable Cell-Penetrating Peptide–Protoporphyrin Conjugate for Targeted Photodynamic Therapy in Vivo. ACS Applied Materials & Interfaces, [PMID:26634784] [10.1021/acsami.5b08637] |
| 27. Shenguang Ge, Lina Zhang, Yan Zhang, Haiyun Liu, Jiadong Huang, Mei Yan, Jinghua Yu. (2015) Electrochemical K-562 cells sensor based on origami paper device for point-of-care testing. TALANTA, [PMID:26459438] [10.1016/j.talanta.2015.05.008] |
| 28. Lu Xu, Xu Xu, Hao Chen, Xingyi Li. (2013) Ocular biocompatibility and tolerance study of biodegradable polymeric micelles in the rabbit eye. COLLOIDS AND SURFACES B-BIOINTERFACES, [PMID:23939422] [10.1016/j.colsurfb.2013.06.047] |
| 29. Shenguang Ge, Weiyan Liu, Lei Ge, Mei Yan, Jixian Yan, Jiadong Huang, Jinghua Yu. (2013) In situ assembly of porous Au-paper electrode and functionalization of magnetic silica nanoparticles with HRP via click chemistry for Microcystin-LR immunoassay. BIOSENSORS & BIOELECTRONICS, [PMID:23728196] [10.1016/j.bios.2013.05.010] |
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| 31. Shen Mingkui, Hou Yan, Xu Shihui, Tan Jun, Zhou Honggang, Miao Qi, Zhang Wanheng, Chen Yazhou, Wang Nana, Wang Yongtao. (2025) Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction. JOURNAL OF NANOBIOTECHNOLOGY, 23 (1): (1-18). [PMID:39825415] [10.1186/s12951-025-03101-x] |
| 32. Jingsong Lu, Yupei Wang, Xiaohan Gao, Ying Li, Neema Ufurahi Pambe, Abdul Fahad, Zeping Jin, zhijun He, Zhenhu Guo, Wensheng Xie, Shi Wang, Xiaodan Sun, Xiumei Wang, Jing Yu, Shenglei Che, Guifeng Zhang, Yen Wei, Lingyun Zhao. (2024) Cancer Nutritional-Immunotherapy with NIR-II Laser Controlled ATP Release Based on Material Repurposing Strategy. Journal of Materials Chemistry B, [PMID:38666407] [10.1039/D4TB00288A] |
| 33. Jiali Li, Weichen Xiong, Jianxi Yang, Weifei Liao, Yihan Gao, Jinwei Chai, Jiena Wu, Shuwen Liu, Xueqing Xu. (2025) Characterization of the first antimicrobial peptide from Sea Seal with potent therapeutic effect in septic mice. BIOCHEMICAL PHARMACOLOGY, [PMID:40147802] [10.1016/j.bcp.2025.116891] |
| 34. Xinyi Zhang, Yuelian Zhang, Huishan Zheng, Xue Yang, Shiqi Zou, Jianmin Chen. (2024) Design, fabrication, and evaluation of antimicrobial sponge microneedles for the transdermal delivery of insulin. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, [PMID:39613270] [10.1016/j.ejpb.2024.114586] |
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| 36. Ziming Zhao, Wenhao Li, Yong Wang, Jiayu An, Qiuchen He, Su Zhan, Feng Zhou. (2025) Introducing microcrystalline cellulose to reinforce the stability of anatase TiO2 for photocatalytic hydrogen production from simulated seawater. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, [10.1016/j.ijhydene.2025.02.190] |
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| 38. Lan Ma, Junjie Liao, Hao Yu, Sheng Shi, Liyun Zhang, Xinchuan Ma, Xin Wen, Xinmei Shi. (2025) Rigid cross-linking: A structural stabilization strategy for enhancing the swelling and compression resistance of GO-based membranes. DESALINATION, [10.1016/j.desal.2025.118671] |
| 39. Li Wang, Kexin Deng, Meng Zhang, Qi Han, Beizhao Chen, Xun Liu, Bei Liu, Yang Yang, Zhongying Wang. (2024) Robust carbon nitride nanosheet interlayered thin-film nanocomposite membrane for enhanced organic solvent nanofiltration. SEPARATION AND PURIFICATION TECHNOLOGY, [10.1016/j.seppur.2024.130485] |
| 40. Zhicheng Le, Yufeng Shou, Renee R. Li, Ling Liu, Runcheng Tan, Christopher John Charles, Zhijia Liu, Yongming Chen, Andy Tay. (2024) Sponge-Like Microneedles Spatially Sequester Chemokines and Deplete Monocytes to Alleviate Inflammatory Skin Disorders. ADVANCED FUNCTIONAL MATERIALS, [10.1002/adfm.202402539] |
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