计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,无RNA酶,用于核酸电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Tris - 硼酸电泳缓冲液(Tris-Borate-EDTA Buffer,简称TBE)是常用的核酸电泳缓冲液。0.5×TBE 工作液组成为:45 mM Tris - 硼酸、1 mM EDTA(pH 8.0),缓冲能力适中,适合分离<2000 bp 的小片段 DNA;0.5~1×TBE 工作液缓冲能力更强,可根据实验需求选用。
TBE缓冲液 (5×, RNase Free) 为5 倍浓缩液,采用 DEPC 处理水配制,主要含 450 mM Tris - 硼酸、10 mM EDTA,主要用于 RNA 电泳,使用时需用 DEPC 处理水稀释至 0.5× 工作液。
本试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
使用前用 DEPC 处理水稀释至 0.5× 工作液即可。
注意事项
1. 10×TBE 储存液稳定性较差,易产生沉淀,推荐选用 5×TBE 浓缩液,储存更稳定。
2. 常规使用 0.5×TBE 作为工作液,如需更强缓冲能力,可选用 0.5~1×TBE 工作液。
3. 实验全程严格避免 RNase 污染。
4. 操作时请穿戴实验服及一次性手套,保障实验安全。
5. 试剂开封后请尽快使用并密封保存,以免影响实验效果。
Tris-Borate-EDTA Buffer (TBE) is a commonly used nucleic acid electrophoresis buffer. The composition of 0.5× TBE working solution is: 45 mM Tris-borate, 1 mM EDTA (pH 8.0), with moderate buffering capacity, suitable for separating small DNA fragments (<2000 bp). The 0.5–1× TBE working solution offers stronger buffering capacity and can be selected according to experimental requirements.
TBE Buffer (5×, RNase Free) is a 5-fold concentrated solution prepared with DEPC-treated water. It primarily contains 450 mM Tris-borate and 10 mM EDTA. It is mainly used for RNA electrophoresis. Before use, dilute to a 0.5× working solution with DEPC-treated water.
This reagent is for research use only. Not for use in diagnostic procedures or other purposes.
Procedure (For Reference Only)
Dilute the product to a 0.5× working solution with DEPC-treated water before use.
Notes
10× TBE stock solution has poor stability and is prone to precipitation. The 5× TBE concentrated solution is recommended for more stable storage.
Routinely use 0.5× TBE as the working solution. For applications requiring stronger buffering capacity, 0.5–1× TBE working solution can be used.
Strictly avoid RNase contamination throughout the entire experimental procedure.
Please wear laboratory appropriate attire and disposable gloves during operation to ensure experimental safety.
Use the reagent as soon as possible after opening and keep it sealed to avoid affecting experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | T1518783 |
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