计算溶液所需的质量、体积或浓度。
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
|---|
有货
为什么选择此级别
EnzymoPure™,天然 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,充氩。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 586 篇同行评审文献引用。
概述
Glucose oxidase is used in the food and beverage industry as a preservative and stabilizer and is commonly derived from the fungus Aspergillus niger. Glucose oxidase can react with intracellular glucose and oxygen (O₂) to produce hydrogen peroxide (H₂O₂) and gluconic acid, which can cut off the nutrition source of cancer cells and consequently inhibit their proliferation.
CATALYSIS : β-D-Glucose + O₂ → D-Glucono-δ-lactone + H₂O₂
SPECIFICATIONS:
Activity: ≥100 U/mg enzyme powder
The activity may increase up to 160%, if the reaction mixture is saturated with oxygen.
Appearance: Yellow powder, lyophilized
Contaminants (Catalase): ≤1 U/mg
Additives: Sodium glutamate
CHARACTERISTICS:
Molecular weight: 140,000 – 160,000 (Gel filtration)
Isoelectric point: 4.3
Km value: 1.9 × 10⁻² M
Inhibitors: Ag⁺, Hg²⁺, Cu²⁺
Opt. pH: 5.0 – 6.5 (Fig. 1)
Opt. temperature: 37°C (Fig. 2)
Stable pH range: 3.0 – 8.0 (40°C, 6hrs) (Fig. 3)
Stable temperature range: below 50°C (pH7.0, 15 min.) (Fig. 4)
Substrate specificity: Table 1
APPLICATIONS:This enzyme is used for the enzymatic determination of glucose in plasma by coupling with mutarotase, and for amylase-activity assay by coupling with α-glucosidase (if maltooligosaccharide or modified starch is used as a substrate) in clinical diagnosis.
Assay Method of Glucose Oxidase
Principle
The appearance of quinoneimine dye formed by coupling with 4-aminopyrine and phenol is measured at 500 nm by spectrophotometry.
Unit Definition
One unit is defined as the enzyme quantity which oxidizes one μ mole of β-D-glucose per minute under the conditions described below.
Reagents
A. Aminoantipyrine Solution (4 mg/ml)
Weigh 0.20 g of 4-aminopyrine and dissolve in 20 ml of deionized water. Fill up to 50 ml with deionized water.
B. Triton X-100 Solution (50 mg/ml)
Weigh 5.0 g of Triton X-100 and dissolve in 30 ml of deionized water with heating. After cooling, fill up to 100 ml with deionized water.
C. Phenol Solution (50 mg/ml)
Weigh 5.0 g of phenol and dissolve in 30 ml of deionized water. Fill up to 100 ml with deionized water.
D. Peroxidase Solution
Dissolve 250 purpurogallin units of Peroxidase in 10 ml of deionized water. Keep in an ice bath. (Expires after 11 days at 2-8°C)
E. 0.1 M Phosphate Buffer (KH₂PO₄-NaOH, pH7.0)
F. Phenol-Buffer Solution
Weigh 1.36 g of KH₂PO₄ and dissolve in 80 ml of deionized water. Add 3 ml of Phenol Solution (C) and 3 ml of Triton X-100 Solution (B), then adjust the pH to 7.0 with 1N NaOH. Fill up to 100 ml with deionized water. This reagent should be used after incubation at 25°C for 24 hours. (Expires after 2 weeks at 25°C)
G. Substrate Solution
Weigh 2.5 mg of D-glucose and dissolve in deionized water. Fill up to 25 ml with deionized water. (Expires after 11 days at room temperature)
H. Enzyme Solution
Weigh approx. 50 mg of Glucose Oxidase and dissolve in chilled 0.1 M Phosphate Buffer (E).
Enzyme Solution should be prepared so that the value of ΔOD/minute becomes in the range of 0.030 ± 0.005.
Procedure
Pipette 2.0 ml of Phenol-Buffer Solution (F), 0.5 ml of Substrate Solution (G), 0.5 ml of Peroxidase Solution (D) and 0.1 ml of Aminoantipyrine Solution (A) into the cuvette (d=10 mm) and keep at 37 ± 0.1°C for 10 minutes. Then, pipette 0.1 ml of Enzyme Solution (H) into the cuvette and mix well immediately. Keep the reaction mixture at 37 ± 0.1°C. Exactly at 2 minutes and 5 minutes after the addition of Enzyme Solution (H), measure the absorbances of the reaction mixture at 500 nm (A5 and A2).
As a blank, pipette 0.1 M Phosphate Buffer (E) into another cuvette (d=10 mm) instead of the Enzyme Solution (H) and take the same procedure described above (Ab5 and Ab2).
Calculation
3: Reaction time
12.88: Reaction time
1/2: Factor based on the fact that two moles of H₂O₂ form one mole of quinoneimine dye
3.2: Final volume of the reaction mixture
0.1: Volume of Enzyme Solution
Dm: Dilution multiple of Enzyme Solution
1.339: Extinction coefficient on the original method using O-dianisidine which is found to be a carcinogenic substance
规格
产品名称
葡萄糖氧化酶 来源于黑曲霉
别名
β-D-葡萄糖 | 氧 1-氧化还原酶
英文别名
BDBM50240803 | glucoside | NCGC00263446-02 | (1->3)-beta-D-glucan | .beta.-D-Glucose | b-d-glucose | beta-D-glucose | GLUCOSE, .BETA.-D | CHEBI:37671 | YC46078 | (1->3)-beta-D-glucopyranan | Pharmakon1600-01300015 | Tabfine 097(HS) | DB02379 | (1->6)-beta
规格或纯度
天然, EnzymoPure™, ≥100 U/mg enzyme powder
生化机理
Glucose oxidase catalyzes the oxidation of beta-D-glucose to D-glucono-delta-lactone and hydrogen peroxide in the presence of molecular oxygen. D-glucono-delta-lactone is sequentially hydrolyzed by lactonase to D-gluconic acid, and the resulting hydrogen peroxide is hydrolyzed by catalase to oxygen and water. The activity shows high specificity to beta-D-glucose, with very low to no activity towards L-glucose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, 4-deoxy-D-glucose, 5-deoxy-D-glucose, 6-deoxy-D-glucose, 3-O-methyl-D-glucose, 4-O-methyl-D-glucose, 6-O-methyl-D-glucose, 4,6-O-benzylidene-D-glucose, 5-thio-5-deoxy-D-glucose, D-mannose, D-allose, D-galactose, D-fructose, D-arabinose, D-xylose, trehalose, melibiose, L-mannomethylose, lactose, sucrose or 1,5-anhydro-D-glucitol.
生物活性
≥100 U/mg enzyme powder
种属
黑曲霉(Aspergillus niger)
来源
天然
CAS编号和信息
9001-37-0
酶学委员会编号
1.1.3.4
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥100 U/mg enzyme powder
复溶
Reconstitute in chilled 0.1 M Phosphate Buffer.
储存条件
-20°C储存,充氩
运输条件
超低温运输
单位定义
One unit is defined as the enzyme quantily which oxidizes oneμmole of β-D-glucose per minute under the conditions described below.
技术文档
高级数据
关联靶点（人）
ASGR1
Tbio
Asialoglycoprotein receptor 1
(30 活性数据)
关联靶点（其它种属）
PC-12 (7051 活性数据)
PYGM
Glycogen phosphorylase, muscle form (1331 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 J2612098 |
分析证书 | G130084 |
| L2515587 |
分析证书 | G130084 |
| L2515602 |
分析证书 | G130084 |
| A2305302 |
分析证书 | G130084 |
| A2305304 |
分析证书 | G130084 |
| A2305353 |
分析证书 | G130084 |
| F2525317 |
分析证书 | G130084 |
| F2525338 |
分析证书 | G130084 |
| A2305303 |
分析证书 | G130084 |
| C2219607 |
分析证书 | G130084 |
| C2219633 |
分析证书 | G130084 |
技术文档和文章
生物酶催化技术及应用
目录： 技术文章
Enzyme Probes
目录： 技术文章
Enzyme Labels in Immunoassays, Signal Amplification, and Multi-Enzyme Detection Platforms: A Functional Overview
目录： 技术文章
From Sucrose to Monosaccharides: Key Enzymatic Nodes and Regulatory Logic in Sugar Conversion
目录： 技术文章
Applications of Enzymatic Protein Crosslinking Systems in Gel Construction and Functional Evaluation
目录： 技术文章
Signal Transduction Mechanism of Graphene Biosensors: From Molecular Recognition to Electrical/Optical Signal Readout and Materials Selection
目录： 技术文章
Experimental Evaluation of the Synergistic Actions of Endoglucanase, CBH I, and β-Glucosidase in Lignocellulosic Saccharification
目录： 技术文章
此产品的引用文献
溶液计算器
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