计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
EnzymoPure™,天然 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 199 篇同行评审文献引用。
概述
细胞定位：Secreted.
序列相似性：Belongs to the glycosyl hydrolase 22 family.
Lysozyme preferentially hydrolyzes the β-1,4 glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. A slightly more limited activity is exhibited toward chitin oligomers. It has a molecular weight of 14,388 daltons. Optimum pH is 9.2. Lysozyme is inhibited by surface active agents such as dodecyl sulfate, alcohol and fatty acids. Imidazole and indole derivatives form inhibitory charged transfer complexes.
Lysozyme is an antimicrobial enzyme that is found in a wide variety of organisms including birds, mammals, plants, insects, and bacteria. The lysozyme of chicken egg white has been most extensively studied.
Stability/Storage: Stable for 3-5 years at 2-8°C. Solutions at pH 4-5 are stable for several weeks refrigerated and for days at ambient temperatures. Store at 2-8°C.
Technical Note: Due to assay differences, 8,000u/mg by Aladdin's assay is equivalent to 50,000u/mg claimed by other suppliers.
Specificity
Lysozyme hydrolyzes the beta-glycosidic linkage between N-acetylmuramic acid and N-acetyl glucosamine in the peptidoglycan of bacterial cell walls and can also bind polymers of N-acetyl glucosamine.
Composition
The structure of lysozyme is consistent under a variety of conditions, making it ideal for crystallography studies. The active site of lysozyme consists of a deep crevice, which divides the protein into two domains linked by an alpha helix. One domain (residues 40 to 85) consists almost entirely of beta-sheet structure, whle the second domain (residues 89-99) is more helical.
Molecular Characteristics
The mature lysozyme of chicken is composed of 128 amino acids. Amino acid sequences of other avian lysozymes are homologous in sequence and differ in only 4 to 20 amino acids . The lysozyme gene in chickens is expressed tissue specifically in the oviduct and in macrophages. Despite there only being one copy of the lysozyme gene, it is regulated differently in the oviduct and macrophages. Regulation of lysozyme in the oviduct utilizes steroid hormones, while a combination of cis-regulatory elements are used during differentiation in macrophages. Transcription is controlled by three enhancers, a complex promoter, and a negative regulatory element
Applications
Nucleic acid preparation
Protein purification from inclusion bodies
Plasmid preparation (to break down membranes and cell wall)
Hydrolysis of chitin
Hydrolysis of bacterial cell walls
Characteristics of Lysozyme
Molecular Weight: 14.3 kDa (Theoretical)
Optimal pH: 6.0-9.0
Isoelectric point: 9.32 (Theoretical)
Extinction Coefficient: 38,940 cm-1 M-1 (Theoretical), E1%,280 = 27.21 (Theoretical)
Active Site Residues: Glutamic acid (E53), Aspartic acid (D70)
Activators: EDTA
Inhibitors: SDS, Alcohols, N-acetyle-D-glucosamine, Oxidizing agents
Lysozyme Assay
Method
The rate of lysis of Micrococcus lysodeikticus is determined as suggested by Shugar (1952). One unit is equal to a decrease in turbidity of 0.001 per minute at 450 nm at pH 7.0 and 25°C under the specified conditions. A wide range of activities are reported for pure lysozyme preparations under these conditions. The specific activity of 8,000 u/mg dw is equivalent to 50,000 u/mg dw claimed by other suppliers.
Reagents
0.1 M Potassium phosphate, pH 7.0
Micrococcus lysodeikticus cells: Prepare by suspending 9 mg of dried Micrococcus lysodeikticus cells in 25 ml of 0.1 M potassium phosphate buffer, pH 7.0. Dilute to a final volume of 30 ml with the same buffer.
Enzyme
Dissolve the enzyme at a concentration of one mg/ml in cold reagent grade water. Keep cool until assay. Immediately prior to assay, dilute to a concentration of 150-500 units/ml with reagent grade water. (The rate should fall between 0.015-0.040 ΔA450/minute).
Procedure
Adjust spectrophotometer to 450 nm and 25°C.
Pipette 2.9 ml of Micrococcus lysodeikticus cell suspension into a cuvette and incubate for 4-5 minutes in order to achieve temperature equilibration and to establish blank rate, if any. Add 0.1 ml of appropriately diluted enzyme to cuvette and record the change in A450 per minute from the initial linear portion of the curve.
Calculation
Biochem/physiol Actions：
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Miscellaneous: Lysozyme C is capable of both hydrolysis and transglycosylation; it also shows a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides.
规格
产品名称
溶菌酶 来源于鸡蛋白(纯化,无盐)
别名
鸡蛋清溶菌酶 | 抗菌肽
英文别名
Mucopeptide N-acetylmuramoylhydrolase | Muramidase | Lysozyme from chicken egg white | LYZ | LYZC | dystrophin
规格或纯度
天然, EnzymoPure™, ≥8,000 units/mg dry weight
生化机理
溶菌酶水解肽聚糖中 N-乙酰胞壁酸和 N-乙酰基-D-葡糖胺残基间的 β(1→4) 键以及壳糊精中 N-乙酰基-D-葡糖胺残基间的 β(1→4)键。革兰氏阳性细胞对该水解作用非常敏感，因为它们的细胞壁含有较高比例的肽聚糖。由于存在外膜以及比例较低的肽聚糖，革兰氏阴性细菌对该水解作用不太敏感。然而，细菌外膜中如果存在与金属离子螯合的 EDTA，那么这些细胞也可能被水解。 该酶在较宽的 pH 范围（6.0 到 9.0）内具有活性。与 pH 为 9.2 时相比，pH 为 6.2 时可在更广的离子强度（0.02 到 0.100M）范围内观察到最大活性。
生物活性
≥8,000 units/mg dry weight
种属
鸡(Chicken)
来源
天然
预测分子量
14.3 kDa
CAS编号和信息
9001-63-2
酶学委员会编号
3.2.1.17
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from salt free.
浓度
≥8,000 units/mg dry weight
储存条件
2-8°C储存
运输条件
低温运输
单位定义
Unit Definition: One Unit is equal to a decrease in turbidity of 0.001 per minute at 450 nm at pH 7.0 and 25°C, using a 0.3mg/ml suspension of Micrococcus lysodeikticus cells as substrate.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 J2610061 |
分析证书 | L128641 |
| J2610063 |
分析证书 | L128641 |
| D2617046 |
分析证书 | L128641 |
| L2501357 |
分析证书 | L128641 |
| L2501415 |
分析证书 | L128641 |
| C2525263 |
分析证书 | L128641 |
| C2525805 |
分析证书 | L128641 |
| D2509695 |
分析证书 | L128641 |
| D2418079 |
分析证书 | L128641 |
| H2330344 |
分析证书 | L128641 |
| H2330345 |
分析证书 | L128641 |
| C2307242 |
分析证书 | L128641 |
| H2209545 |
分析证书 | L128641 |
| H2209546 |
分析证书 | L128641 |
技术文档和文章
溶菌酶的结构特点及其应用进展
目录： 技术文章
His-Tag Protein Purification: Common Troubleshooting Questions and Practical Solutions
目录： 常见问题(FAQs)
AmpC β-Lactamases (Cephalosporinases): Mechanisms of Production, Diagnostic Pathways, and Research and Clinical Applications
目录： 技术文章
Review of Chymotrypsin: Enzymological Properties, Catalytic Mechanism, and Research Applications
目录： 技术文章
Functional System Overview of Cellular Metabolic Enzymes in Energy Metabolism, Redox, and Signal Transduction
目录： 技术文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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