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ACC在生物体内催化乙酰辅酶A羧化生成丙二酰辅酶A，是脂肪酸和许多次生代谢产物合成的关键酶。ACC的活性在一定程度上决定了脂肪酸的合成速度和含油量的高低。 测定原理：ACC能够催化乙酰辅酶A、NaHCO₃和ATP生成丙二酰辅酶A、ATP和无机磷，通过钼酸铵定磷法测定无机磷的增加量来测定ACC活性。
Acetyl-CoA Carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to produce malonyl-CoA within living organisms. It is a key enzyme in the synthesis of fatty acids and many secondary metabolites. The activity of ACC largely determines the rate of fatty acid synthesis and the level of oil content. Assay Principle: ACC catalyzes the formation of malonyl-CoA, ADP, and inorganic phosphate from acetyl-CoA, NaHCO₃, and ATP. The activity of ACC is determined by measuring the increase in inorganic phosphate using the ammonium molybdate colorimetric method.
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