Mouse anti Human Actin Regulatory Protein CAPG/MCP Antibody, Monoclonal (K52030_15B12), could be used for WB, IHC and so on. Application: WB: 1/1000 IHC: 1/200 Protein Function: Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Actin regulatory protein CAP G antibody | Actin regulatory protein CAP-G antibody | AFCP antibody | Capg antibody | CAPG_HUMAN antibody | Capping actin protein gelsolin like antibody | Capping protein (actin filament) gelsolin like antibody | Capping prot
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
小鼠(Mouse)
特异性
Actin Regulatory Protein CAPG/MCP
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human Actin Regulatory Protein CAPG/MCP (AA 191-270).
阳性对照
WB: THP-1, U937, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human lung tissue.
Actin Regulatory Protein CAPG/MCP Mouse mAb (Ab087281) - Western Blot All lanes: Actin Regulatory Protein CAPG/MCP Mouse mAb (Ab087281) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 86.7 s
Actin Regulatory Protein CAPG/MCP Mouse mAb (Ab087281) - IHC IHC analysis of lung tissue by Actin Regulatory Protein CAPG/MCP Mouse mAb (Ab087281).IHC was performed using sections of the formalin-fixed paraffin-embedded lung tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with Actin Regulatory Protein CAPG/MCP Mouse mAb (Ab087281) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Macrophages are positively stained at the cytoplasm.
ZJ24F0708914
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