Recombinant alpha Tubulin Antibody

Name: Recombinant alpha Tubulin Antibody
SKU: Ab088465
Availability: InStock

货号: Ab088465

COA

有货

级别和纯度: 重组 ? ExactAb™ ? 已验证 ? 高性能 ? 见COA

应用

IF/ICC, IHC, WB

宿主种属

兔(Rabbit)

种属反应性

人(Human), 大鼠(Rat), 小鼠(Mouse)

亚型

Rabbit IgG

偶联

Unconjugated

规格

库存

价格

数量

10μl

Ab088465-10μl

现货

¥149.90

50μL

Ab088465-50μl

现货

¥449.90

100μL

Ab088465-100μl

期货

¥697.40

1ml

Ab088465-1ml

期货

¥6,799.90

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概述规格储存与运输图片应用技术文档高级数据关联靶点（人）质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器常见问题

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为什么选择此级别

ExactAb™,已验证,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Rabbit anti Human alpha Tubulin Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

规格

产品名称

Recombinant alpha Tubulin Antibody

别名

英文别名

规格或纯度

重组, ExactAb™, 已验证, 高性能, 见COA

生化机理

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Post-translational: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

宿主种属

兔(Rabbit)

特异性

alpha Tubulin

种属反应性

人(Human),大鼠(Rat),小鼠(Mouse)

免疫原

A synthetic peptide derived from human alpha 4A Tubulin (AA 10-51).

阳性对照

WB: Mouse brain, HeLa, Hep G2, Jurkat, HEK293, NIH/3T3 and C6 lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa, Hep G2 cells.

偶联

Unconjugated

克隆类型

重组抗体

Format

Whole IgG

轻链亚型

kappa

纯化方法

Protein A purified

浓度

见COA

分子类型

抗体

产品线

生物产品

储存与运输

物理外观

Liquid

储存缓冲液

10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4.

防腐剂

0.02% Sodium azide

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

图片

Western Blot: Recombinant alpha Tubulin Antibody (Ab088465)

Recombinant alpha Tubulin Antibody (Ab088465) - Western Blot
All lanes: Recombinant alpha Tubulin Antibody (Ab088465) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 82.0 s

IHC: Recombinant alpha Tubulin Antibody (Ab088465)

Recombinant alpha Tubulin Antibody (Ab088465) - IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Recombinant alpha Tubulin Antibody (Ab088465).