ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Alpha Tubulin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Post-translational
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Recombinant Alpha Tubulin Antibody (Ab088473)- Western Blot
All lanes: Recombinant Alpha Tubulin Antibody (Ab088473) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 53 kDa
Exposure time: 0.5 s
Recombinant Alpha Tubulin Antibody (Ab088473) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant Alpha Tubulin Antibody (Ab088473). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Alpha Tubulin Antibody (Ab088473) – IF/ICC
Immunocytochemistry analysis of Alpha Tubulin (green) in HeLa cells using Recombinant Alpha Tubulin Antibody (Ab088473), and cells were counterstained with DAPI (blue).
1/500-1/1000
1/50 - 1/100
1/50-1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0939459 |
分析证书 | Ab088473 |
| ZJ24F1011782 |
分析证书 | Ab088473 |
| ZJ26F0737171 |
分析证书 | Ab088473 |
| ZJ23F1101583 |
分析证书 | Ab088473 |
| ZJ23F1101582 |
分析证书 | Ab088473 |
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