Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
宿主种属
兔(Rabbit)
特异性
Angiopoietin 1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Angiopoietin 1 (AA 250-300).
阳性对照
WB: HeLa, MDA-MB-231, RAW 264.7 and C6 cell lysates.
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Angiopoietin 1 Antibody (Ab088793) - Western Blot All lanes: Recombinant Angiopoietin 1 Antibody (Ab088793) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 58 kDa Observed band size: 63, 67 kDa Angiopoietin 1 primarily exists as a full-length, biologically active protein that binds to the TIE2 receptor, promoting blood vessel maturation and stability. It can be found in glycosylated and deglycosylated forms, which influence its stability and function. Additionally, Angiopoietin 1 exists as both soluble and membrane-bound forms, allowing for local signaling variations. Exposure time: 100.0 s
ZJ25F0217629
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