Rabbit anti Human Cytokeratin 8 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 0.01-2 µg/mL IHC: 5-20 µg/mL ICC/IF: 5-20 µg/mL Protein Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Recombinant Cytokeratin 8 expressed in E.coli (AA 92-393)
阳性对照
WB: A431, A549, HeLa, Hep G2 and MCF7 cell lysates. IHC: Human liver cancer, tonsil, prostate, thyroid cancer, kidney and stomach tissues. ICC/IF: MCF7 cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified anand Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
Cytokeratin 8 Antibody (Ab099041) - Western Blot All lanes: Cytokeratin 8 Antibody (Ab099041) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 37-55 kDa Exposure time: 0.3 s
Cytokeratin 8 Antibody (Ab099041) - IF/ICC FITC staining on IF Samples: Human MCF7 cells Primary Ab: 20 μg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 20 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Tonsil Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Thyroid cancer Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cytokeratin 8 Antibody (Ab099041) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10 µg/mL Cytokeratin 8 Antibody (Ab099041) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.