Rabbit anti Human Dnmt1 Antibody, Recombinant, could be used for WB, IHC and so on.
规格
产品名称
Recombinant Dnmt1 Antibody
别名
DNA甲基转移酶1抗体
英文别名
AIM | CXXC9 | CXXC9CXXC finger protein 9 | CXXC-type zinc finger protein 9 | DNA (cytosine-5-)-methyltransferase 1 | DNA (cytosine-5)-methyltransferase 1 | DNA methyltransferase 1 | DNA methyltransferase HsaI | DNA MTase HsaI | DNMT | EC 2.1.1.37 | DNMT1
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
宿主种属
兔(Rabbit)
特异性
DNMT1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human DNMT1 (AA 1500-1616)
阳性对照
WB: HeLa and Jurkat cell lysates. IHC: Rat spleen, Human lung large cell cancer, Human glioblastoma, Mouse lung tissues.
Recombinant Dnmt1 Antibody (Ab100220) - Western Blot All lanes: Recombinant Dnmt1 Antibody (Ab100220) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 183 kDa Observed band size: 182, 360 kDa The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19173286). Exposure time: 56.4 s
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Rat spleen, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
Recombinant Dnmt1 Antibody (Ab100220) - IHC Immunohistochemical analysis of paraffin-embedded Mouse lung, using Recombinant Dnmt1 Antibody (Ab100220) at 1/100 dilution.
ZJ24F1215792
危险品化学品经营许可证（带存储）