Mouse monoclonal (7H12) to E Cadherin, could be used for WB, IHC and Flow.
Application
WB: 1/500-1/2000
IHC: 1/200-1/1000
Flow: 1/200-1/400
ELISA: 1/10000
Purity
Protein G purified.
Protein Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
E Cadherin Mouse mAb (Ab100725) - Western Blot
All lanes: E Cadherin Mouse mAb (Ab100725) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 135 kDa
Observed band size: 112&124 kDa
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human gastric tissues using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - Flow Cytometry
Flow cytometric analysis of HeLa cells using E Cadherin Mouse mAb (Ab100725) (green) and negative control (purple).
1/500-1/2000
1/200-1/1000
1/200-1/400
1/10000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1011949 |
分析证书 | Ab100725 |
| ZJ23F0700709 |
分析证书 | Ab100725 |
| ZJ23F0700712 |
分析证书 | Ab100725 |
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