Recombinant protein corresponding to Human E-Cadherin
阳性对照
WB: MCF-7 and LNCaP whole cell lysates ICC/IF: MCF-7 cells
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
AB03/2B8
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
图片
E-Cadherin Mouse mAb (Ab186487) - Western Blot All lanes: E-Cadherin Mouse Antibody (Ab186487) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 97 kDa Observed band size: 109 kDa Exposure time: 20.6 s
E-Cadherin Mouse mAb (Ab186487) - ICC/IF IF analysis of E-Cadherin (Red) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with E-Cadherin Mouse mAb (Ab186487) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
ZJ24F0809917
危险品化学品经营许可证（带存储）