Recombinant antibody to FXR1, could be used for WB, IF, ICC and IHC. Application: WB: 1/500 – 1/1000 IF/ICC: 1/50 – 1/200 IHC: 1/50 – 1/100 Function RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.
规格
产品名称
Recombinant FXR1 Antibody
别名
脆性X相关蛋白1/脆性X智力低下综合征相关蛋白1抗体 | 重组 FXR1 抗体
英文别名
Fragile X mental retardation autosomal homolog 1 antibody | Fragile X mental retardation syndrome related protein 1 antibody | Fragile X mental retardation syndrome-related protein 1 antibody | FXR1 antibody | FXR1_HUMAN antibody | FXR1P antibody | hFXR1p
规格或纯度
ExactAb™, Validated, 重组, 0.7 mg/mL
宿主种属
兔(Rabbit)
特异性
FXR1
应用
WB,IF,ICC,IHC
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human FXR1 (AA 42-56).
阳性对照
WB: HeLa,MCF7,HEK293,A549 lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Recombinant FXR1 Antibody (Ab104281) - Western Blot All lanes: Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 70 kDa Observed band size: 70-80 kDa
Recombinant FXR1 Antibody (Ab104281) - IF IF analysis of HeLa cells labeling FXR1 with purified Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
Recombinant FXR1 Antibody (Ab104281) - IF IF analysis of FXR1 (green) in HeLa cells using Recombinant FXR1 Antibody (Ab104281) and cells were counterstained with DAPI (blue).
Recombinant FXR1 Antibody (Ab104281) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant FXR1 Antibody (Ab104281). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.