Rabbit anti Human G3BP Antibody, Recombinant, could be used for WB, IF/ICC and IHC. Application: WB: 1/500 – 1/1000 IF/ICC: 1/50 – 1/200 IHC: 1/50 – 1/100 Protein Function May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
Recombinant G3BP Antibody (Ab104334) - Western Blot All lanes: Recombinant G3BP Antibody (Ab104334) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 68 kDa Observed band size: 64 kDa
Recombinant G3BP Antibody (Ab104334) - ICC/IF IF analysis of G3BP (green) in Hela using G3BP antibody (Ab104334), and cells were counterstained with DAPI (blue).
Recombinant G3BP Antibody (Ab104334) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using G3BP antibody (Ab104334) . High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.