ExactAb™,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human GBP4 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.
Application:
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
Flow Cytometry: 20 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function:
Binds GTP, GDP and GMP. Hydrolyzes GTP very efficiently; GDP rather than GMP is the major reaction product. Plays a role in erythroid differentiation.
GBP4 Antibody (Ab104956) - Western Blot
All lanes: GBP4 Antibody (Ab104956) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 72 kDa
Exposure time: 6.5 s
GBP4 Antibody (Ab104956) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 20 µg/mL GBP4 Antibody (Ab104956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
GBP4 Antibody (Ab104956) - Flow Cytometry
Human A431 cell was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100, then stained with 20 µg/mL GBP4 Antibody (Ab104956) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.
GBP4 Antibody (Ab104956) - IF/ICC
IF analysis of GBP4 Antibody (Ab104956) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GBP4 Antibody (Ab104956) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
5-20 µg/mL
20μg/mL
0.01-2 µg/mL
2.5 µg/mL
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0505220 |
分析证书 | Ab104956 |
| ZJ24F0505221 |
分析证书 | Ab104956 |
| ZJ24F0505222 |
分析证书 | Ab104956 |
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