ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Histone H3 Antibody, Recombinant (RR692), could be used for WB, IHC, Flow, IP and so on.
Application:
WB: 1/1000 - 1/5000
IHC: 1/800 - 1/1600
Flow: 1/50 - 1/200
IP: 1/100
Recombinant Histone H3 Antibody (Ab107980) - Western Blot
All lanes: Recombinant Histone H3 Antibody (Ab107980) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15, 17 kDa
Exposure time: 7.1 s
Recombinant Histone H3 Antibody (Ab107980) - Western Blot
All lanes: Recombinant Histone H3 Antibody (Ab107980) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15, 17 kDa
Exposure time: 33.2 s
Recombinant Histone H3 Antibody (Ab107980) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling Histone H3 with Recombinant Histone H3 Antibody (Ab107980) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Histone H3 Antibody (Ab107980) - IP
Histone H3 was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant Histone H3 Antibody (Ab107980) at 100 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant Histone H3 Antibody (Ab107980) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant Histone H3 Antibody (Ab107980) in HeLa whole cell lysate
Lane 3: Hela whole cell lysate, 10 μg (input)
Recombinant Histone H3 Antibody (Ab107980) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Histone H3 Antibody (Ab107980) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Histone H3 Antibody (Ab107980) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
1/800 - 1/1600
1/50 - 1/200
1/800 - 1/1600
1/100
1/1000 - 1/5000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F1201978 |
分析证书 | Ab107980 |
| ZJ23F1201979 |
分析证书 | Ab107980 |
| ZJ25F0319503 |
分析证书 | Ab107980 |
| ZJ24F1114499 |
分析证书 | Ab107980 |
| ZJ24F0202982 |
分析证书 | Ab107980 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 High Performance 级别指南 →
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