H3 histone family member E pseudogene antibody | H3 histone family, member A antibody | H3/A antibody | H31_HUMAN antibody | H3F3 antibody | H3FA antibody | Hist1h3a antibody | HIST1H3B antibody | HIST1H3C antibody | HIST1H3D antibody | HIST1H3E antibody
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
宿主种属
兔(Rabbit)
特异性
Histone H3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Histone H3.1 (AA 100-136).
阳性对照
WB: HeLa, K562, SH-SY5Y, MDA-MB-231, NIH/3T3 and C6 cell lysates. IHC: Rat thymus, Human esophageal carcinoma, tonsil, colon cancer, Mouse spleen, brain tissue. IF/ICC: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Histone H3 Antibody (Ab213824) - Western Blot All lanes: Recombinant Histone H3 Antibody (Ab213824) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 15, 17 kDa The expression profile and molecular weight observed may be due to post-translational modifications. Exposure time: 1.0 s
Recombinant Histone H3 Antibody (Ab213824) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Histone H3 Antibody (Ab213824).
Recombinant Histone H3 Antibody (Ab213824) - IHC Immunohistochemical analysis of paraffin-embedded Rat thymus, using Recombinant Histone H3 Antibody (Ab213824) at 1/200 dilution.
Recombinant Histone H3 Antibody (Ab213824) - IHC Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using Recombinant Histone H3 Antibody (Ab213824) at 1/200 dilution.
Recombinant Histone H3 Antibody (Ab213824) - IHC Immunohistochemical analysis of paraffin-embedded Human tonsil, using Recombinant Histone H3 Antibody (Ab213824) at 1/200 dilution.
Recombinant Histone H3 Antibody (Ab213824) - IHC Immunohistochemical analysis of paraffin-embedded Human colon cancer, using Recombinant Histone H3 Antibody (Ab213824) at 1/200 dilution.
Recombinant Histone H3 Antibody (Ab213824) - IHC Immunohistochemical analysis of paraffin-embedded Mouse spleen, using Recombinant Histone H3 Antibody (Ab213824) at 1/200 dilution.
Recombinant Histone H3 Antibody (Ab213824) - IHC Immunohistochemical analysis of paraffin-embedded mouse brain, using Recombinant Histone H3 Antibody (Ab213824) at 1/200 dilution.