MLRW | MHC Class II DR | MHC class II antigen DRA | MHC cell surface glycoprotein | major histocompatibility complex, class II, DR alpha | HLA-DRA1 | HLA-DRA | HLA-DR | HLADR | HLA class II histocompatibility antigen, DR alpha chain | histocompatibility a
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
宿主种属
兔(Rabbit)
特异性
HLA-DRA
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human HLA DRA (AA 150-190).
阳性对照
WB: Raji and Ramos cell lysates. IHC: Human lung tissue. ICC/IF: Raji cells.
Recombinant HLA-DR Antibody (Ab108253) - Western Blot All lanes: Recombinant HLA-DR Antibody (Ab108253) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 32 kDa Exposure time: 14.6 s
Recombinant HLA-DR Antibody (Ab108253) - ICC/IF Immunofluorescent analysis of Raji cells, using Recombinant HLA-DR Antibody (Ab108253).
Recombinant HLA-DR Antibody (Ab108253) - IHC Immunohistochemical analysis of paraffin-embedded human lung, using Recombinant HLA-DR Antibody (Ab108253).
ZJ24F1012276
危险品化学品经营许可证（带存储）