Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) - Western Blot All lanes: Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 211 kDa Observed band size: 120 kDa Exposure time: 15.8 s
Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) - IHC Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903).
Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) - IF/ICC IF analysis of Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).